基于orthogonal design优选墓头回中总多糖含量测定条件  

Optimization of Determination Conditions of Total Polysaccharide Content in Herba Patriniae Based on Orthogonal Design

作　　者：王嘉琛 张莉丹 王伊楠 郭宇航 董征艳 姚隆丹 段秀俊 WANG Jia-chen;ZHANG Li-dan;WANG Yi-nan;GUO Yu-hang;DONG Zheng-yan;YAO Long-dan;DUAN Xiu-jun(Department of Traditional Chinese Medicine,Shanxi Pharmaceutical Vocational College,Shanxi Taiyuan 030031;Shanxi University of Chinese Medicine,Shanxi Taiyuan 030031 China)

机构地区：[1]山西药科职业学院,山西太原030031 [2]山西中医药大学,山西太原030031

出　　处：《广州化工》2021年第5期109-112,共4页GuangZhou Chemical Industry

基　　金：山西省地方药材标准项目(2012013A);山西地标对照药材研究项目(2016001C)。

摘　　要：优化墓头回的显色条件,并测定不同产地提取液中总多糖的含量。考察多个显色因素对墓头回中总多糖含量测定的影响并在最佳显色条件下对10批药材进行总多糖含量测定。结果表明墓头回总多糖在苯酚浓度为9%,水浴温度为70℃,加热时间为10 min,静置时间为5 min,在最大吸收波长489 nm为最佳显色条件。该方法操作简便、准确、稳定可靠,可用于墓头回药粉中多糖含量的测定。The color development conditions of tomb head back were optimized and the content of total polysaccharides in extracts from different producing areas was determined.The influence of multiple color development factors on the determination of total polysaccharide content in Herba Patriniae was investigated and the total polysaccharide content of 10 batches of medicinal materials was determined under the best color development conditions.The results showed that the total polysaccharide in Herba Patrinia was 9%phenol,the temperature of the water bath was 70℃,the heating time was 10 min,the standing time was 5 min,and the maximum absorption wavelength of 489 nm was the best color development condition.The method is simple,accurate,stable and reliable,and can be used to determine the content of polysaccharides in the tomb back powder.

关 键 词：优选条件 墓头回 总多糖 含量测定 正交试验 

分 类 号：R284.1[医药卫生—中药学]