抗条锈病小偃麦附加系CH366的FISH鉴定  

FISH Identification of Wheat-Thinopyrum intermedium Addition Line CH366 with Resistance to Stripe Rust

作　　者：郑瑗 李欣[2] 贾举庆[2] 郭慧娟 雷梦林 张树伟 常利芳 畅志坚 乔麟轶 陈芳 张晓军 ZHENG Yuan;LI Xin;JIA Juqing;GUO Huijuan;LEI Menglin;ZHANG Shuwei;CHANG Lifang;CHANG Zhijian;QIAO Linyi;CHEN Fang;ZHANG Xiaojun(College of Bio-engineering,Shanxi University,Taiyuan 030006,China;College of Agronomy,Shanxi Agricultural University,Shanxi Key Laboratory of Crop Genetics and Molecular Improvement,Taiyuan 030031,China;Center for Agricultural Genetic Resources Research,Shanxi Agricultural University,Key Laboratory of Crop Gene Resources andGermplasm Enhancement on Loess Plateau,Ministry of Agriculture,Taiyuan 030031,China)

机构地区：[1]山西大学生物工程学院,山西太原030006 [2]山西农业大学农学院,作物遗传与分子改良山西省重点实验室,山西太原030031 [3]山西农业大学农业基因资源研究中心,农业部黄土高原作物基因资源与种质创制重点实验室,山西太原030031

出　　处：《山西农业科学》2021年第3期278-280,284,共4页Journal of Shanxi Agricultural Sciences

基　　金：山西省重点研发计划项目(201903D211003,201803D221018-5,201803D421020,201903D211073);山西省农业科学院农业科技创新研究课题(YCX2018D2YS01);山西省重点科技创新平台项目(201605D151002)。

摘　　要：利用小麦-中间偃麦草部分双二倍体TAI7045与普通小麦晋太170进行亚远缘杂交选育的小偃麦新种质CH366高抗小麦条锈菌条中32、条中33和条中34号生理小种,为了追踪其抗条锈病基因来源,对CH366及其亲本接种条锈菌生理小种,并对CH366进行了荧光原位杂交(FISH)鉴定。结果表明,CH366和TAI7045对条锈病表现为近免疫和免疫,其小麦亲本晋太170则表现为高感,证明CH366的条锈病抗性来源于TAI7045;FISH鉴定表明,CH366中含有44条染色体,其中,42条来源于晋太170,另2条来源于TAI7045的中间偃麦草染色体。CH366是一个小麦-中间偃麦草二体异附加系,可作为小麦抗条锈病遗传改良的中间亲本。Thinopyrum intermedium,one of the important gene sources for wheat genetic improvement,can improve significantly the disease resistance of cultivated wheat by introducing its resistance gene into wheat backgroud.CH366,a novel germplasm with resistance to stripe rust of Pst CYR32,CYR33 and CYR34,derived from sub-wide cross of wheat-Thinopyrum intermedium partial diploid TAI7045 and common wheat Jintai 170.To trace the origin of stripe rust resistance genes,the resistance of CH366 and its parents were identified by artificial inoculation with stripe rust physiological races,and CH366 was identified by fluorescence in situ hybridization(FISH).The results showed that CH366 and TAI7045 showed near immunity and immunity while Jintai 170 showed high sensitivity to stripe rust.It suggested that the resistance of CH366 might come from TAI7045.FISH results showed that CH366 contained 44 chromosomes,of which 42 were derived from Jintai 170 and the other 2 from Thinopyrum intermedium chromosomes of TAI7045.As a result,CH366 is a wheat-Thinopyrum intermedius disomic alien additional line,which can be used as an intermediate parent for genetic improvement of wheat resistance to stripe rust.

关 键 词：小麦 中间偃麦草 附加系 FISH 抗条锈病 

分 类 号：S512.9[农业科学—作物学]