单细胞拉曼光谱在环境解磷微生物中的应用研究  

Application of single-cell Raman spectroscopy to investigate phosphate-solubilizing bacteria in environment

作　　者：林绍敏 李弘哲 崔丽[1] LIN Shaomin;LI Hongzhe;CUI Li(Key Lab of Urban Environment and Health,Institute of Urban Environment,Chinese Academy of Sciences,Xiamen 361021,China;College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou 350000,China)

机构地区：[1]中国科学院城市环境研究所中科院城市环境与健康重点实验室,福建厦门361021 [2]福建农林大学生命科学学院,福建福州350000

出　　处：《磷肥与复肥》2021年第2期1-4,共4页Phosphate & Compound Fertilizer

基　　金：国家重点研发计划项目子课题(2017YFD0200201)。

摘　　要：利用解磷微生物活化土壤中大量固定态磷是维持农业可持续发展和缓解磷资源危机的重要途径。对新近发展的单细胞拉曼光谱-重水同位素标记联用技术解析高活性解磷菌的原理,及其在原位土壤中识别高效解磷微生物的应用研究进行介绍;与多种传统解磷微生物研究方法进行对比,讨论不同方法的优缺点。对未来单细胞拉曼光谱与其他技术的结合及其在解磷微生物上的深入研究进行展望。Activation of the large amount of phosphorus(P)fixed in soil by PSB is an effective way for sustainable agronomic use of P and mitigating the world P crisis.The principle of single-cell Raman spectroscopy combined with D2O isotope labeling(Raman-D2O)technology for the analysis of highly active PSB,and their application for in probing highly efficient PSB in situ soils are introduced.Single-cell Raman-D2O technology is further compared with multiple conventional methods for PSB studies,and the advantages and disadvantages of different approaches are discussed.Finally,the potential coupling of single-cell Raman spectroscopy with other technologies for an in-depth PSB investigation is prospected.

关 键 词：解磷微生物 单细胞拉曼光谱 重水同位素标记 

分 类 号：TQ041.7[化学工程] S132[农业科学—农业基础科学]