Establishment of A Simple and Efficient Agrobacterium-mediated Genetic Transformation System to Chinese Cabbage(Brassica rapa L.ssp.pekinensis)  

作　　者：Xiaonan Li Haiyan Li Yuzhu Zhao Peixuan Zong Zongxiang Zhan Zhongyun Piao 

机构地区：[1]Department of Horticulture,Shenyang Agricultural University,Shenyang,Liaoning 110866,China

出　　处：《Horticultural Plant Journal》2021年第2期117-128,共12页园艺学报（英文版）

基　　金：the National key research and Development Program(Grant No.2017YFD0101802);the National Natural Science Foundation of China(Grant Nos.31772326 and 31701930);China Postdoctoral Science Foundation(Grant Nos.2016M601345 and 2019T120219).

摘　　要：Chinese cabbage,belonging to Brassica rapa species,is an important vegetable in Eastern Asia.It is well known that Chinese cabbage is quite recalcitrant to genetic transformation and the transgenic frequency is generally low.The lack of an efficient and stable genetic transformation system for Chinese cabbage has largely limited related gene functional studies.In this study,we firstly developed a regeneration system for Chinese cabbage by optimizing numerous factors,with 93.50%regeneration rate on average.Based on this,a simple and efficient Agrobacteriummediated genetic transformation methodwas established,without pre-culture procedure and concentration adjustment of hormone and AgNO_(3) in co-cultivation and selection media.Using this system,transformants could be obtained within 3.5–4.0 months.Average transformation frequency is up to 10.83%.The establishment of this simple and efficient genetic transformation method paved the way for further gene editing and functional studies in Chinese cabbage.

关 键 词：Chinese cabbage Agrobacterium tumefaciens genetic transformation 

分 类 号：S634.1[农业科学—蔬菜学]