Transcriptome Analysis of Female and Male Henosepilachna vigintioctopunctata(Coleoptera:Coccinellidae)Identifies Sex-related Genes  

作　　者：Wei Guo Zhuoqi Liu Jiaqi Yu Jing Lü Xiaoguo Jiao Lan Kong Chunxiao Yang Huipeng Pan 

机构地区：[1]Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province,Engineering Technology Research Center of Agricultural Pest Biocontrol of Guangdong Province,South China Agricultural University,Guangzhou 510642,China [2]State Key Laboratory of Biocatalysis and Enzyme Engineering,Center for Behavioral Ecology&Evolution,School of Life Sciences,Hubei University,Wuhan 430062,China [3]Department of Computer Science,Eastern Kentucky University,Richmond KY 40475,USA [4]State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources,South China Agricultural University,Guangzhou 510642,China

出　　处：《Horticultural Plant Journal》2021年第2期142-148,共7页园艺学报（英文版）

基　　金：the National Key R and DProgram of China(Grant No.2017YFD0200900);the Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme(2017).

摘　　要：Henosepilachna vigintioctopunctata is an emerging horticultural pest of Solanaceae plants in Asia.Here,we employed transcriptome sequencing of three female and three male H.vigintioctopunctata libraries to identify sex-related genes.We recorded 281599294 bp of clean reads,and de novo assembly generated 85206 unigenes,with N_(50) of 1311 bp and average length of 763 bp.We found 5002 genes highly expressed in males and 2179 genes highly expressed in females.Expression profiles of six unigenes specific to females,six unigenes specific to males,and ten unigenes common to both sexes were verified using semi-quantitive RT-PCR;results showed expression levels of these 22 unigenes were relatively consistent with the transcriptome analysis.Using all unigenes as references,we identified 6657 putative simple sequence repeats.The transcriptome sequences and gene expression profiles of female and male H.vigintioctopunctata help explore genetic mechanisms of sexual dimorphism.

关 键 词：Henosepilachna vigintioctopunctata SEX RNA-seq transcriptome annotation differential expression 

分 类 号：S433.5[农业科学—农业昆虫与害虫防治]