DNA修复基因APE1 Asp148Glu多态性与中国西南地区女性宫颈癌风险及预后相关  

Polymorphism of DNA repair gene APE1(Asp148Glu)is associated with risk and prognosis of cervical cancer in women of Southwest China

作　　者：许文婧[1,2] 张诗珩 王东[1] XU Wenjing;ZHANG Shiheng;WANG Dong(Cancer Center,Daping Hospital,Army Medical University(Third Military Medical University),Chongqing,400042;Department of Oncology,Nanqiaosi Branch of Chongqing Hospital of Traditional Chinese Medicine,Chongqing,400021,China)

机构地区：[1]陆军军医大学(第三军医大学)大坪医院肿瘤中心,重庆400042 [2]重庆市中医院南桥寺院部肿瘤科,重庆400021

出　　处：《第三军医大学学报》2021年第5期418-424,共7页Journal of Third Military Medical University

基　　金：国家自然科学基金面上项目(30670628,30801367)。

摘　　要：目的探讨中国西南地区女性DNA修复基因APE1多态性与宫颈癌风险和预后的相关性。方法调查2007年1月至2014年7月在陆军军医大学大坪医院就诊的290例宫颈癌患者的临床特征,以同期290例体检的健康志愿者为对照;并对患者的总生存期进行随访,使用PCR对APE1(Asp148Glu和-141T/G)进行基因分型。利用χ2检验和Hardy-Weinberg平衡分析病例与对照之间基因的多态性;采用多元Logistic回归模型评估基因多态性与宫颈癌风险的相关性,用Kaplan-Meier法评估患者的总生存与不同基因型的关系。结果与APE1 Asp148 Asp/Asp相比,Glu/Glu基因型显示出患宫颈癌的风险更低(OR=0.542,P<0.05),特别是具有以下临床特征的患者:生育次数<2次(OR=0.345,P<0.05),初潮年龄≥14岁(OR=0.522,P<0.05),未绝经患者(OR=0.324,P<0.05),绝经年龄≥45岁(OR=0.495,P<0.05),病理类型为鳞状细胞癌(OR=0.597,P<0.05)。而含有Glu的基因型具有更多的生存获益(P=0.001),但是APE1 pro-141T/G多态性与宫颈癌风险没有显著关联。结论纯合子APE1 Asp148 Glu/Glu基因型有较低的子宫颈癌风险及较好的预后。Objective To investigate whether the polymorphism of the DNA repair gene APE1(Asp148 Glu)is associated with the risk and prognosis of cervical cancer in women of Southwest China.Methods We studied the clinical features of 290 woman with cervical cancer diagnosed in Daping hospital from January 2007 to July 2014.And another 290 healthy volunteers who took physical examination during the same period were recruited and served as normal control.The patients were followed up for the total survival.PCR-CTPP was used detect the genotype of APE1(Asp148 Glu and-141 T/G)in the patients and normal control.Chi-square test was applied to assess the distribution of individual clinical characteristics,and Hardy-Weinberg equilibrium was used to analyze the polymorphisms of these genes between the case and control.Multivariate logistic regression models were to assess the association of these polymorphisms with risk of cervical cancer.And Kaplan-Meier survival analysis was used to evaluate the relationship of overall survivals(OS)in different genotypes.Results The Glu/Glu genotype showed a lower risk of cervical cancer(OR=0.542,P<0.05)when compared with the APE1 Asp148 Asp/Asp genotype,especially for the individuals with following features:childbirth<2(OR=0.345,P<0.05),menarche older than 14 years old(OR=0.522,P<0.05),non-menopause(OR=0.324,P<0.05),age of menopause≥45 years old(OR=0.495,P<0.05)or squamous carcinoma(OR=0.597,P<0.05).And those carrying Glu genotype had a higher OS benefit than those carrying the APE1 Asp148 Asp genotypes(P=0.001).However,there was no significant association between the APE1 pro-141 T/G polymorphism and risk of cervical cancer.Conclusion Homozygous APE1148 Glu/Glu genotype has a reduced risk and a better prognosis of cervical cancer.

关 键 词：宫颈癌 APE1 单核苷酸多态性 癌症风险 癌症预后 

分 类 号：R195.4[医药卫生—卫生统计学] R394.5[医药卫生—卫生事业管理]