重组藻蓝蛋白与水稻类囊体膜之间光能传递的初步研究  

A preliminary study on light energy transfer between recombinant phycocyanin and rice thylakoid membrane

作　　者：孙德光 郭雅琳 章峰 尚孟慧 臧晓南[1] 张学成[1] SUN Deguang;GUO Yalin;ZHANG Feng;SHANG Menghui;ZANG Xiaonan;ZHANG Xuecheng(The Key Laboratory of Marine Genetics and Breeding of Ministry of Education,Ocean University of China,Qingdao 266003,PR China)

机构地区：[1]海洋生物遗传育种教育部重点实验室中国海洋大学,山东青岛266003

出　　处：《海洋湖沼通报》2021年第1期71-76,共6页Transactions of Oceanology and Limnology

基　　金：国家自然科学基金项目(31872555)资助。

摘　　要：为了研究藻蓝蛋白和高等植物类囊体膜之间的光能传递,本实验将诱导表达的重组藻蓝蛋白和水稻类囊体混合孵育后,检测其77K荧光发射光谱以及类囊体膜的电子传递速率和光化学量子产量,结果表明重组藻蓝蛋白和水稻类囊体膜光系统I之间存在能量传递使得光化学量子产量提高。本研究为利用藻胆蛋白构建新型光合系统提供了理论基础和实验依据。The light energy transfer between phycocyanin and rice thylakoid membrane were determined in this study.The recombinant phycocyanin was purified and incubated with rice thylakoid with 77 K fluorescent emission spectrum,light energy electron transfer efficiency(ETR)and photochemical quantum yield(Y)determined then.The results indicated that energy transfer between the recombinant phycocyanin and the rice thylakoid membrane photosystem I existed,which caused an increase in photochemical quantum yield.Our findings provided a foundation for the construction of a novel photosynthetic system using phycobiliprotein.

关 键 词：重组藻蓝蛋白 光能传递 类囊体膜 77K荧光 光化学量子产率 

分 类 号：Q71[生物学—分子生物学]