无机砷As(Ⅲ、Ⅴ)对牟氏角毛藻生长、叶绿素荧光特性及基因组DNA甲基化水平的影响  

Effects of inorganic arsenic As(Ⅲ,Ⅴ)exposure on the growth,chlorophyll fluorescence parameters and genomic DNA methylation of Chaetoceros mulleri

作　　者：孙琰晴 陈亚男[1,2] 郝明梅 李斌 徐东 姚殿志[4] 陈丽竹 李焕军 孙春晓 由丽萍 苏博 邹荣婕 Yanqing Sun;Ya’nan Chen;Mingmei Hao;Bin Li;Dong Xu;Dianzhi Yao;Lizhu Chen;Huanjun Li;Chunxiao Sun;Liping You;Bo Su;Rongjie Zou(National Demonstration Center for Experimental Fisheries Science Education,Centre for Research on Environmental Ecology and Fish Nutrition(CREEFN)of Ministry of Agriculture,International Research Center for Marine Biosciences of Ministry of Science and Technology,Shanghai Ocean University,Shanghai 201306,PR China;Shandong Key Laboratory of Marine Ecology Restoration,Shandong Marine Resource and Environment Research Institute,Yantai,Shandong 264006,PR China;Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,PR China;Longkou Municipal Ocean and Fisheries Administration,Longkou,Shan dong 265700,PR China)

机构地区：[1]上海海洋大学,水产科学国家级实验教学示范中心,农业部鱼类营养与环境生态研究中心,海洋生物科学国际联合研究中心(中国科学技术部),上海201306 [2]山东省海洋资源与环境研究院山东省海洋生态修复重点实验室,山东烟台264006 [3]中国水产科学研究院黄海水产研究所,山东青岛266071 [4]龙口市海洋与渔业局,山东龙口265700

出　　处：《海洋湖沼通报》2021年第1期85-90,共6页Transactions of Oceanology and Limnology

基　　金：国家自然科学基金(41206094);山东省自然科学基金(ZR2017MD025)“无机砷在大型褐藻海带中的富集转化及毒理效应研究”。

摘　　要：以AsO_(2)^(-)和HAsO_(4)^(2-)分别暴露处理牟氏角毛藻(Chaetoceros mulleri)研究As(Ⅲ、Ⅴ)毒性作用,As(Ⅲ)浓度梯度为0、10、50、100、300和500μmol/L,As(Ⅴ)浓度梯度为0、30、60、120、300和600μmol/L,分别在第1、3、7d取样,研究不同浓度的As(Ⅲ、Ⅴ)对藻细胞的生长、叶绿素荧光特性以及基因组DNA甲基化水平的影响。结果显示,在3d时100μmol/L As(Ⅲ)组牟氏角毛藻生长率降为0,两个高浓度组1-7d的生长率均小于0,且均明显低于对照(P<0.05);而一定浓度As(Ⅴ)暴露初期会提高藻细胞生长率。低浓度As(Ⅲ)暴露组牟氏角毛藻的荧光参数Fv/Fm和Yield与对照组差异不显著(P>0.05),两个高浓度组荧光参数的值则均显著低于对照;而As(Ⅴ)对牟氏角毛藻的PSII系统影响不显著。一定浓度范围内的As(Ⅲ、Ⅴ)可以引起牟氏角毛藻DNA甲基化水平明显升高,而长期暴露高浓度As(Ⅲ)后藻类DNA甲基化水平与对照差异不大。综上可知,As(Ⅲ)对牟氏角毛藻的生长、叶绿素荧光特性的毒害作用大于As(Ⅴ),而对DNA甲基化的影响与As(Ⅴ)相似。The effects of inorganic arsenic As(Ⅲ,Ⅴ)at different concentrations on the growth,chlorophyll fluorescence characteristics and total methylation level of Chaetoceros mulleri were studied.The exposure concentrations of As(Ⅲ,Ⅴ)were 0,10,50,100,300 and 500μmol/L AsO_(2)^(-)and 0,30,60,120,300 and 600μmol/L HAsO_(4)^(2-),respectively.The microalgal cells were sampled at 1,3 and 7 d of exposure.The results showed that the exposure to 100μmol/L As(Ⅲ)inhibited the growth of C.mulleri completely at 3 d.Two groups of higher exposure concentrations showed clearly lower growth rates(both below zero)than the control(P<0.05).When C.mulleri was exposed to a certain concentration range of As(Ⅴ),the growth rate increased at initial experiment stage.The chlorophyll fluorescence characteristics(Fv/Fm)and yield were not significantly different between two lower concentrations of As(Ⅲ)and the control(P>0.05),but the count of two groups of higher exposure concentrations were significantly lower than the control.As(V)had no significant effect on the PSII system of C.mulleri.The exposure to a certain concentration range of As(Ⅲ,Ⅴ)increased the total methylation level of C.mulleri genome.This effect was not found at higher concentrations of As(Ⅲ)after a long time of exposure.In summary,the toxic effect of As(Ⅲ)on the growth and chlorophyll fluorescence characteristics of C.mulleri was greater than that of As(Ⅴ),but similar on the genome methylation.

关 键 词：无机砷 牟氏角毛藻 生长率 叶绿素荧光特性 DNA总甲基化水平 

分 类 号：X55[环境科学与工程—环境工程]