体外环境下增强纤维蛋白凝胶稳定性的初步研究  

Strengthen stability of fibrin glue in vitro:a initinal study

作　　者：骆晓婷 曾奕明[1] Luo Xiaoting;Zeng Yiming(Department of Respiratory and Critical Care Medicine,the Second Affiliated Hospital of Fujian Medical University,Respiratory Medicine Center of Fujian Province,Quanzhou 362000,China)

机构地区：[1]福建医科大学附属第二医院呼吸与危重症医学科,福建省呼吸医学中心,泉州362000

出　　处：《国际呼吸杂志》2020年第24期1874-1878,共5页International Journal of Respiration

摘　　要：目的探讨在体外环境下不同浓度的抑肽酶及氨甲环酸对纤维蛋白凝胶溶解速度的影响,以获得稳定性良好的纤维蛋白凝胶,以便更好地应用于临床。方法制作不同特性的纤维蛋白凝胶:抑肽酶浓度按15000、20000、25000 kIU/ml,分为A、B、C组;氨甲环酸浓度按10、30、50 g/L,分为Ⅰ、Ⅱ、Ⅲ组;设立无抑制剂的对照组;以抑肽酶25000 kIU/ml+氨甲环酸50 g/L制作抗纤溶剂联合的纤维蛋白凝胶,标记为U组。将各组纤维蛋白凝胶置于37℃恒温水槽中,每隔24小时测量溶解的体积。结果在同一浓度纤维蛋白原中,抑肽酶、氨甲环酸均可延缓纤维蛋白凝胶的溶解速度(F_(抑肽酶组)=502.379,F_(氨甲环酸组)=632.235,P值均<0.05)。将各组每日剩余体积进行组间多重比较,对照组与A组、B组、C组比较,A组与B组、C组比较,差异均有统计学意义(P值均<0.05);B组与C组比较,差异无统计学意义;对照组与Ⅰ组、Ⅱ组、Ⅲ组比较,Ⅰ组与Ⅱ组、Ⅲ组比较,Ⅱ组与Ⅲ组比较,差异均有统计学意义(P值均<0.05)。与单用抑肽酶或氨甲环酸相比,联合抑肽酶+氨甲环酸可明显延缓凝胶溶解速度(F值分别为366.417、262.533,P值均<0.05)。结论高浓度的抑肽酶、氨甲环酸均可增强纤维蛋白凝胶的稳定性。在同一浓度纤维蛋白原中,抑肽酶<20000 kIU/ml,酶浓度的增加可延缓溶解速度;而抑肽酶>25000 kIU/ml,凝胶溶解速度无明显变化;氨甲环酸浓度增加可延缓凝胶的溶解速度;联合抑肽酶+氨甲环酸延缓纤维蛋白凝胶溶解的作用优于单用抑肽酶或氨甲环酸。Objective To investigate the effects of different concentrations of aprotinin and tranexamic acid on dissolution rate of fibrin glue in vitro,so as to make more stable fibrin glue to be applicated in clinic setting.Methods Different concentrations of the aprotinin as 15000,20000 and 25000 kIU/ml were added individually into group A,group B and group C,and different concentrations of tranexamic acid as 10,30 and 50 g/L were added into groupⅠ,groupⅡand groupⅢ.The control group was set up as adding neither of them.Group U was labeled by adding 25000 kIU/ml aprotinin and 50 g/L tranexamic acid.All of the groups were put in a 37℃water bath for 24 hours.The dissolving volume was measured every 24 hours.Results In the same condition of the fibrinogen,either aprotinin or tranexamic acid slowed down the dissolution rate of the fibrin glue(F_(ap)=502.379,F_(TXA)=632.235,all P<0.05).The multiple comparison results showed that there was statistical significance in daily residual volume between control group and group A,B,C,or between group A and group B,C(all P<0.05),but there was no statistical significance in that between group B and group C.There was statistical significance in daily residual volume between control group and groupⅠ,Ⅱ,Ⅲ,or between groupⅠand groupⅡ,Ⅲ,or between groupⅡand groupⅢ(all P<0.05).Comparing with single dose group,the combination use of the aprotinin and tranexamic acid slowed down the dissolution rate of the fibrin glue(F=366.417,262.533,respectively,all P<0.05).Conclusions Higher dose of aprotinin and tranexamic acid can strengthen the stability of the fibrin glue.In the same condition of the fibrinogen with aprotinin<20000 kIU/ml,the higher dose of the aprotinin can decrease the dissolution rate of the glue.No significant changes have been proved when the aprotinin concentration is higher than 25000 kIU/ml.On the other side,increased dose of tranexamic acid can result in decreased dissolution rate in a dose response manner.The combination use of the aprotinin and tranexami

关 键 词：纤维蛋白凝胶 抑肽酶 氨甲环酸 

分 类 号：R318.08[医药卫生—生物医学工程]