RT-PCR和NASBA方法检测HIV-1感染者病毒载量结果分析  

Analysis of viral load in HIV-1 infected patients by RT-PCR and NASBA

作　　者：严亚军[1] 梁科[1] 冯玲[1] 杨蓉蓉[1] YAN Ya-jun;LIANG Ke;FENG Ling;YANG Rong-rong(Department of Infectious Diseases,Zhongnan Hospital,Wuhan University,Wuhan,Hubei 430071,China)

机构地区：[1]武汉大学中南医院感染科,湖北武汉430071

出　　处：《热带医学杂志》2020年第12期1537-1539,共3页Journal of Tropical Medicine

基　　金：国家自然科学基金(81000758);中国性病艾滋病防治协会·艾伯维妇幼关爱及预防母婴传播基金(PMTCT2019-010)。

摘　　要：目的比较逆转录酶-聚合酶链反应(RT-PCR)和核酸序列依赖性扩增反应(NASBA)两种方法检测人类免疫缺陷病毒1型(HIV-1)感染者病毒载量的相关性和一致性。方法采用RT-PCR和NASBA方法平行检测30例新发现的HIV-1感染者病毒载量,并将检测结果进行统计分析。结果 RT-PCR和NASBA方法检测30例新发现HIV-1感染者病毒载量均值分别为(5.188±0.703)Log/mL和(5.135±0.903)Log/mL,差异无统计学意义(P>0.05)。相关性分析显示两种方法检测HIV-1感染者病毒载量呈显著直线相关(r=0.811,P<0.000 1),Bland-Altman分析显示两种方法检测HIV-1感染者病毒载量具有较好的一致性。结论 RT-PCR和NASBA方法检测HIV-1感染者病毒载量具有较好的相关性及一致性。Objective The correlation and consistency of viral load in human immunodeficiency virus 1(HIV-1) infected patients were compared between RT-PCR and NASBA.Methods The viral load of 30 newly discovered HIV-1 infected patients was detected by RT-PCR and NASBA in parallel,and the results were statistically analyzed.Results The mean viral load of 30 newly discovered HIV-1 infected patients was(5.188±0.703) Log/mL and(5.135±0.903) Log/mL by RTPCR and NASBA respectively.Paired t test analysis showed that the there was no statistically significance between the two methods(P>0.05).Correlation analysis showed that there was a significant linear correlation between the two methods(P<0.000 1).Bland-Altman analysis showed that the results of two methods for detecting viral load in HIV-1 infected patients were consistent.Conclusion RT-PCR and NASBA had good correlation and consistency in the detection of viral load in HIV-1 infected patients.

关 键 词：人类免疫缺陷病毒1型 RT-PCR方法 NASBA方法 病毒载量 

分 类 号：R512.91[医药卫生—内科学]