基于转录组研究补光对设施‘红地球’葡萄萌芽的影响  

Effects of supplementary light on the bud burst of‘Red Globe’grape under protected cultivation based on transcriptome sequencing

作　　者：刘帅 徐伟荣[1] 张亚红[1] 刘鑫 郭松涛 胡莉 LIU Shuai;XU Weirong;ZHANG Yahong;LIU Xin;GUO Songtao;HU Li(School of Agronomy,Ningxia University,Yinchuan 750021,Ningxia,China)

机构地区：[1]宁夏大学农学院,银川750021

出　　处：《果树学报》2021年第3期305-317,共13页Journal of Fruit Science

基　　金：国家自然科学基金(31360493)。

摘　　要：【目的】探究不同光质补光对设施‘红地球’葡萄冬芽萌发的影响。【方法】以8年生,红地球'葡萄为试验材料,采用4种不同光质补光(红蓝光2:1、蓝光、红光、白光)处理‘红地球’冬芽,以不补光为对照,进行生理指标测定和转录组学分析。【结果】红蓝光2:1处理下芽萌发最快,其展叶率及可溶性糖、总蛋白和H2O2含量均高于其他处理。利用FPKM计算基因表达量,以差异表达倍数|log2fold changespl、P-adjust<0.05为筛选条件,共获得1423个差异表达基因,包括上调基因309个,下调基因1114个,其中红蓝光2:1、蓝光、红光、白光处理与对照之间的差异基因数目分别为1051、880、836和325个;GO分析发现差异基因涉及代谢过程、细胞过程、结合和催化活性等。KEGG分析显示,差异基因主要富集在植物与真菌互作、植物激素信号转导、内质网蛋白加工、植物MAPK信号通路等途径;其中植物信号转导途径中生长素、细胞分裂素、赤霉素、脱落酸、乙烯、油菜素内酯和茉莉酸信号转导相关基因在红蓝光2:1处理下表达显著。根据富集结果随机选取9个差异表达基因进行qRT-PCR验证,基因的表达趋势与转录测序结果基本一致。【结论】不同光质补光均加快了葡萄芽的萌发,红蓝光2:1可作为葡萄芽萌发的理想光质;植物激素信号转导通路中SAUR.A-ARR.GIDKPYR/PYL、PP2C基因的差异表达,是各处理葡萄芽萌发差异的重要原因。[Objective]The early-maturing grape cultivation in greenhouse extends the market supply period of table grape,and improves the economic benefit of grape industry significantly.‘Red Globe’grape is the main cultivar of grape for the greenhouse culture in Ningxia.However,due to aging of the film and the dust on the surface of the film,the grapes are exposed to low density of light in the greenhouse,resulting in the reduction of fruit quality and economic income.Light can regulate the gene expression,substance metabolism and morphological fbmiation of plants.Artificial light is an efficient measure to improve the light conditions in the greenhouse.At present,researches on the effects of the light quality on grape mainly focus on plant growth,development and physiological metabolisms.In order to clarify the effect mechanism of different light quality supplementation on the bud burst of grape in the molecular level,transcriptome sequencing was used to analyze the light response related genes in the buds exposed to different light quality.【Methods】The experiment started in Helan Horticultural Industrial Park,experimental base of Ningxia University,on March 25,2019.The experimental materials were eight-year-old‘Red Globe’grapes with a growing space of 0.8 mx 1.5 m in the solar greenhouse.Four different light quality treatments were red light(H),blue light(L),white light(B),red blue light(red light:blue light=2∶1,HL),the control was no supplementary light(CK).The intensity of supplementary light was 200μmol·m-2·s-1,and the duration of supplementary light was 4h per day.On April 16,2019,the buds in the leaf development stage were counted.The third and the fourth buds on the base of the annual branches with consistent growth in the leaf-developing stage were taken.3 groups were randomly selected for each treatment,and 10 buds were taken for each group.The buds were immediately put in liquid nitrogen after stripping from the branches and stored in the refrigerator at-80℃.The soluble sugar,total protein,H2O

关 键 词：‘红地球’葡萄 光质 转录组测序 差异表达基因 

分 类 号：S663.1[农业科学—果树学]