不同益生菌发酵后白术总黄酮与总多糖含量测定研究  

Research on the content of total flavonoids and total polysaccharids in Atractmlodis macrocephalae rhizoma after fermentation

作　　者：叶思勇[1] 张宁 朱荣刚[1] Ye Siyong;Zhang Ning;Zhu Ronggang(Department of Pharmacy,the Second People's Hospital of Ji'nan,Ji'nan 250001,China;Department of Quality Management,China Resources Shandong Pharmaceutical Co.,Ltd,Ji'nan 250118,China)

机构地区：[1]济南市第二人民医院药剂科,250001 [2]华润山东医药有限公司质量管理部,济南250118

出　　处：《国际中医中药杂志》2020年第12期1145-1148,共4页International Journal of Traditional Chinese Medicine

摘　　要：目的建立白术发酵样品中总黄酮和总多糖的含量测定方法,考察白术发酵前后总黄酮和总多糖的含量变化,为白术发酵的深入研究开发提供科学依据。方法利用酵母菌、双歧杆菌、乳酸菌对白术进行发酵。采用紫外分光光度法在波长500 nm处,测定发酵白术中总黄酮含量。以苯酚-硫酸法显色,在490 nm处测定发酵白术中总多糖含量。结果白术经酵母菌、双歧杆菌、乳酸菌发酵后,总黄酮含量分别提高了2.29、1.81、1.87倍,酵母菌发酵后总多糖含量降低47.89%,双歧杆菌发酵后总多糖含量提高36.41%,乳酸菌发酵后总多糖含量提高14.73%。结论白术经酵母菌、双歧杆菌、乳酸发酵后,其总黄酮、总多糖含量变化显著,可提高有效成分相对含量。Objective To establish the content determination and study the content changes of total flavonoids and total polysaccharids in fermented Atractmlodis macrocephalae rhizoma with different probiotics.Methods Atractmlodis macrocephalae rhizoma was fermented with three probiotics:yeast,lactic acid bacteria,bifidocacterium.The contents of total flavonoids and total polysaccharids in fermentation samples were determined by using UV-visible spectrophotometry at the wavelength of 510 nm and 490 nm respectively.Results After the fermentation with different probiotics,the content of total flavonoids increased by 2.29 times,1.81 times and 1.87 times respectively,and the content of total polysaccharids in fermented Atractmlodis macrocephalae rhizoma increased by 36.41%and 14.73%respectively.Conclusion After the fermentation with yeast,bifidobacterium and lactic acid,the content of total flavonoids and total polysaccharides of Atractmlodis macrocephalae rhizoma changed significantly,which could improve the relative content of effective ingradient.

关 键 词：白术 益生菌 黄酮类 多糖类 炮制(中药) 

分 类 号：R284.1[医药卫生—中药学]