针灸对克罗恩病患者结肠黏膜基因表达谱的影响  

Effect of Acupuncture-moxibustion on Colon Mucosal Gene Expression Profile in Crohn’s Disease Patients

作　　者：翁志军[1,2] 汪迪 郑寒丹 施茵[1,2] 刘雅楠[1,2] 包春辉[1,2] 陆嫄[1,2] 吴焕淦[1,2] 刘慧荣 马晓芃[1,2] 吴璐一[3] 黄艳[1,2] WENG Zhi-jun;WANG Di;ZHENG Han-dan;SHI Yin;LIU Ya-nan;BAO Chun-hui;LU Yuan;WU Huan-gan;LIU Hui-rong;MA Xiao-peng;WU Lu-yi;HUANG Yan(Shanghai Research Institute of Acupuncture and Meridian,Shanghai 200030,China;Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China;Key Laboratory of Acupuncture-moxibustion and Immunological Effects,Shanghai University of Traditional Chinese Medicine,Shanghai 200030,China)

机构地区：[1]上海市针灸经络研究所,上海200030 [2]上海中医药大学附属岳阳中西医结合医院,上海200437 [3]上海中医药大学针灸免疫效应重点研究室,上海200030

出　　处：《上海针灸杂志》2021年第3期269-278,共10页Shanghai Journal of Acupuncture and Moxibustion

基　　金：国家自然科学基金青年项目(81904303);上海市卫生局中医药科研基金资助项目(2014LQ065A);上海市科技启明星计划基金资助项目(16QA1403400);上海市优秀学术带头人计划资助项目(17XD1403400)。

摘　　要：目的从基因表达谱的角度,探讨针灸治疗克罗恩病(CD)的效应机制。方法受试者签署知情同意书进行结肠镜检查,收集CD患者和健康者结肠黏膜。采用基因芯片技术检测健康志愿者及CD患者针灸治疗前后结肠黏膜的基因表达谱,筛选出针灸治疗CD的差异表达基因,结合生物信息学方法,对差异表达的基因进行GO和Pathway分析。结果针灸治疗CD后结肠黏膜组织炎症反应较治疗前明显减轻;与健康者比较,CD结肠黏膜差异表达基因共有3449个(上调2250个,下调1199个)。与治疗前比较,针灸治疗后CD结肠黏膜差异表达基因共有1926个(上调536个,下调1390个),其中CD结肠黏膜差异表达的基因能被针灸逆转的有388个(上调62个,下调326个);针灸可调控的CD中差异表达基因与疾病密切相关的生物功能主要有转录因子STAT磷酸化、ATP酶活性、B细胞介导的免疫、自然杀伤细胞相关免疫等,相关的通路主要有Toll-like受体信号通路、自噬调节系统、自然杀伤细胞介导的细胞毒作用。结论针灸能有效减轻CD患者结肠炎症反应,影响CD结肠黏膜组织异常的基因表达谱,其差异表达基因主要涉及细胞周期、免疫炎症反应、细胞自噬等生物学过程。Objective To discuss the action mechanism of acupuncture-moxibustion in treating Crohn’s disease(CD)from the perspective of gene expression profile.Method The subjects all signed the informed consent form to receive colonoscopy and colon mucosa was collected from the CD patients and healthy subjects.Gene microarray technique was used to detect the colon mucosal gene expression profile in the healthy volunteers and CD patients before and after acupuncture-moxibustion treatment.The differentially expressed genes in acupuncture-moxibustion treatment of CD were screened to undergo GO and Pathway analyses in combination with bioinformatic method.Result Significant reduction of inflammatory response was found in colon mucosal tissues in CD after acupuncture-moxibustion treatment.Compared with the healthy volunteers,there were 3449 differentially expressed genes in CD colon mucosa(2250 up-regulated and 1199 down-regulated).The expressions of 1926 genes(536 up-regulated and 1390 down-regulated)incolon mucosa differed after acupuncture-moxibustion treatment of CD compared with the pre-treatment state,and 388(62 up-regulated and 326 down-regulated)out of the differentially expressed genes in CD colon mucosa were reversed by acupuncture-moxibustion;the CD-related differentially expressed genes that could be modulated by acupuncture-moxibustion were mainly associated with biological functions including transcription factor STAT phosphorylation,ATPase activity,B cell-mediated immunity and natural killer cell-related immunity,and the relevant pathways mainly were toll-like receptor signaling pathway,autophagy regulation system,natural killer cell-mediated cytotoxicity.Conclusion Acupuncture-moxibustion can effectively reduce colonic inflammatory response in CD patients,influence the abnormal gene expression profile in CD colon mucosal tissues,and the differentially expressed genes are mainly involved in biological processes such as cell cycle,immune inflammatory response and cellular autophagy.

关 键 词：针灸疗法 克罗恩病 基因表达谱 信号通路 免疫 

分 类 号：R246.1[医药卫生—针灸推拿学]