杂交籼稻稻瘟病抗性分子标记辅助育种的探索与实践  

Exploration and practice on improvement of indica hybrid rice blast resistance by using molecular marker assisted breeding

作　　者：李育红[1,2,3] 吴云雨 张小祥[1,2,3] 肖宁 余玲[1,2,3] 刘广青 潘存红[1,3] 周长海 王闯[1,2,3] 黄年生 蔡跃[1,3] 季红娟 王志平[1,2,3] 戴正元 李爱宏[1,3] LI Yuhong;WU Yunyu;ZHANG Xiaoxiang;XIAO Ning;YU Ling;LIU Guangqing;PAN Cunhong;ZHOU Changhai;WANg Chuang;HUANg Niansheng;CAI Yue;JI Hongjuan;WANG Zhiping;DAI Zhengyuan;LI Aihong(Lixiahe Region Agricultural Research Institue of Jiangsu Province/Nanjing Sub-Center of National Rice Improve Center,Yangzhou 225007,China;Yangzhou Bojinyuan Agricultural Science Development Company Limited,Yangzhou 225007,China;Jiangsu Collaborative Innovation Center for Modern Industrial Technology of Grain Crops,Yangzhou 225009,China)

机构地区：[1]江苏里下河地区农业科学研究所/国家水稻改良中心南京分中心扬州试验站,江苏扬州225007 [2]扬州金源农业科学发展有限公司,江苏扬州225007 [3]江苏省粮食作物现代产业技术协同创新中心,江苏扬州225009

出　　处：《扬州大学学报（农业与生命科学版）》2020年第6期22-27,33,共7页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：江苏省自然科学基金资助项目(BK20181216);扬州市现代农业项目(YZ2019035);国家现代农业(水稻产业)产业技术体系建设专项(CARS-01-45)。

摘　　要：利用具有广谱和持久抗性的抗稻瘟病基因培育抗病品种是控制稻瘟病危害最有效途径之一。通过分子标记辅助选择将广谱抗稻瘟病基因Pigm导入温敏核不育系隆科638S,育成新的抗稻瘟病温敏核不育系扬籼6S;利用分子标记将华占的稻瘟病抗性基因Pi2、Pi54、Pid3、Pita和Pib等导入辐恢838中,育成新的抗稻瘟病恢复系扬恢1512,与华占相比,抗性提高,结实率和千粒重显著增加。以扬籼6S作母本,以扬恢1512作父本,配制杂交组合,育成两系杂交籼稻新组合扬两优612,其稻瘟病抗性综合指数为2.8,表现中抗。扬籼6S和扬恢1512的成功选育,为培育抗稻瘟病杂交稻新品种提供了抗病新种质;而扬两优612的成功选育,则为杂交水稻稻瘟病抗性育种实践提供了实例。Using broad-spectrum and durable resistance genes in rice breeding is one of the most effective ways to control rice blast disease in rice production.In this study,the broad-spectrum blast resistance gene Pigm was introduced into the thermo-sensitive male sterile(TMS)line Longke 638Sby using molecular marker-assisted selection,and a new blast resistance TMS line Yangxian 6Swas developed.The Pi2,Pi54,Pid3,Pitaand Pibfrom Huazhan were introduced into Fuhui 838by using molecular marker-assisted selection and a new rice blast resistance restorer line Yanghui 1512was bred.Compared to Huzhan,Yanghui 1512displayed higher resistant level to blast disease,especially the seed setting rate and thousand-grain weight were increased significantly.Using Yangxian 6Sas the female parent and Yanghui 1512as the male parent,we produced a new two-line hybrid indica rice combination Yangliangyou 612,which had a comprehensive blast resistance index of 2.8and reached medium resistance level.The successful breeding of Yangxian 6Sand Yanghui 1512provided new resistance germplasm for breeding new rice blast resistant hybrid varieties,and the successful breeding of Yangliangyou 612provided breeding practice examples for developing blast resistant hybrid rice.

关 键 词：杂交籼稻 分子标记辅助选择 抗稻瘟病基因 

分 类 号：S511[农业科学—作物学]