江苏省小麦品种(系)硬度基因分布特点解析  

Analysis on the distribution of allelic variation for grain hardness of wheat in Jiangsu province

作　　者：胡文静[1,2,3] 吴宏亚 董亚超[3] 吴迪 高德荣 HU Wenjing;WU Hongya;DONG Yachao;WU Di;GAO Derong(Lixiahe Region Institute of Agriculture Sciences of Jiangsu Province/Key Laboratory of Wheat Biology and Genetic Improvement for Low&Middle Yangtze Valley,Ministry of Agriculture and Rural Affairs,Yangzhou 225007,China;Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops,Yangzhou University,Yangzhou 225009,China;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China;Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/College of Agriculture,Yangzhou University,Yangzhou 225009,China)

机构地区：[1]江苏里下河地区农业科学研究所/农业农村部长江中下游小麦生物学与遗传育种重点实验室,江苏扬州225007 [2]扬州大学/江苏省粮食作物现代产业技术协同创新中心,江苏扬州225009 [3]中国农业科学院作物科学研究所,北京100081 [4]扬州大学农学院/江苏省作物遗传生理重点实验室/植物功能基因组学教育部重点实验室/江苏省作物基因组学和分子育种重点实验室,江苏扬州225009

出　　处：《扬州大学学报（农业与生命科学版）》2020年第6期28-33,共6页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家自然科学基金资助项目(32071999);国家现代农业产业技术体系建设专项(CARS-03-03B、CARS-3-2-11);江苏省现代农业重点项目(BE2017340)。

摘　　要：以江苏省近20年育成的111份小麦品种(系)为供试材料,对其籽粒硬度Pina-D1和Pinb-D1等位变异进行KASP标记检测,研究其分布频率。结果表明:江苏省小麦软质基因组合类型Pina-D1a/Pinb-D1a占54.95%,硬质基因组合Pina-D1a/Pinb-D1b和Pina-D1b/Pinb-D1a分别占38.74%和6.31%。近20年扬州地区育成小麦品种(系)以软质类型基因组合为主,南京和镇江地区育成小麦品种(系)是软质兼顾硬质类型基因组合,随着种植区域由南向北,软质小麦基因组合类型的小麦品种(系)数量比例逐渐下降,淮阴和徐州地区育成的绝大多数小麦品种(系)属于硬质类型基因组合Pina-D1a/Pinb-D1b。Grain hardness is one of the main objectives of wheat quality breeding.To clarify the distribution of puroindoline-D1(Pin-D1)gene in wheat cultivars(lines)from Jiangsu province in recent 20years,a total of 111wheat cultivars(lines)were used to detect the Pina-D1and Pinb-D1genesusing KASP(kompetitive allele specific PCR)markers.The result indicated that Pina-D1a/Pinb-D1awas the dominant gene combination for wheat in Jiangsu province with frequency of 54.95%,and Pina-D1a/Pinb-D1b gene combination was the dominant type for hard wheat with frequency of 38.74%,and 6.31%wheat cultivars(lines)carried Pina-D1b/Pinb-D1agene combination.In recent 20years,soft gene combination mainly distributed in Yangzhou,and the soft gene combination was as many as the hard gene combination in Nanjing and Zhenjiang.The proportion of soft gene combination was gradually decreased,and most of the wheat cultivars(lines)in Huaiyin and Xuzhou carried Pina-D1a/Pinb-D1b gene combination for hard wheat.This study identified the distribution frequencies of Pin-D1gene in wheat cultivars(lines)of different areas in Jiangsu province,and provides materials and information for wheat quality breeding of this province.

关 键 词：小麦 puroindoline-D1(Pin-D1)基因 KASP 软质麦 硬质麦 品质育种 

分 类 号：S512[农业科学—作物学]