福建主栽非洲菊品种遗传多样性ISSR分析  

ISSR Analysis on the Genetic Diversity of Main Cultivars of Gerbera Jamesonii Bolus in Fujian Province

作　　者：夏朝水 江斌 邓永生 陈玮婷 曹奕鸯 XIA Chao-shui;JIANG Bin;DENG Yong-sheng;CHEN Wei-ting;CAO Yi-yang(Sanming Academy of Agricaltural Sciences,Sanming,Fujian 365051,China;Qingliu County Forestry Bureau,Sanming,Fujian 365300,China)

机构地区：[1]三明市农业科学研究院,福建三明365051 [2]清流县林业局,福建三明365300

出　　处：《福建农业科技》2021年第1期9-14,共6页Fujian Agricultural Science and Technology

基　　金：福建省花卉品种引进与研发创新项目(明林造〔2015〕40号);中央引导地方科技发展专项(2019L3025)。

摘　　要：利用ISSR技术对35份福建主栽非洲菊资源进行遗传多样性分析。从100条ISSR引物中筛选出多态性好的15条随机引物,分别对35份非洲菊资源基因组DNA进行扩增,共扩增得到78条清晰可辨条带,其中多态性条带70条,多态位点百分比达89.7%。利用DPS软件统计分析,结果表明,各品种间的遗传相似系数介于0.2968~0.7500,当阈值为0.4200时,可将35个非洲菊分成4类。当遗传相似系数为0.4914时,第I类非洲菊又可分为4个亚类。聚类结果表明,花心颜色和重瓣类型可以作为非洲菊亲缘关系分类的一种依据,同一搜集来源的品种有聚类到一起的趋势,这与农艺性状的聚类结果有一定的对应关系。Genetic diversity of 35 major cultivars of Gerbera jamesonii Bolus in Fujian was analyzed by ISSR technology.15 random primers with good polymorphism were selected from 100 ISSR primers,and were amplified on the genomic DNA of 35 Gerbera jamesonii Bolus.A total of 78 clear and distinguishable bands were obtained,of which 70 were of polymorphism,and the percentage of polymorphic sites reached 89.7%.The DPS software was used for the statistical analysis,and the results showed that the genetic similarity coefficients among the varieties ranged from 0.2968 to 0.7500.When the threshold value was 0.4200,the 35 Gerbera jamesonii Bolus could be divided into four categories.When the genetic similarity coefficient was 0.4914,the Class I Gerbera jamesonii Bolus could be further divided into four subclasses.The clustering results showed that the color of flower heart and the type of double petals could be used as a basis for the classification of genetic relationship of Gerbera jamesonii Bolus,and the varieties from the same source tended to cluster together,which had a certain corresponding relationship with the clustering results of agronomic traits.

关 键 词：非洲菊 种质资源 简单重复序列间扩增 遗传多样性 

分 类 号：S682.1[农业科学—观赏园艺]