桔梗多糖对桔梗皂苷D在体肠吸收的影响  

Effects of Platycodon grandiflorum polysaccharides on in situ intestinal absorption of platycodin D

作　　者：陈青青 任蓉蓉 张青青 杨晔 CHEN Qing-qing;REN Rong-rong;ZHANG Qing-qing;YANG Ye(College of Pharmacy,Anhui University of Chinese Medicine,Hefei 230012,China;Ministry of Education Key Laboratory for Xin’an Medicine,Hefei 230012,China;Anhui Provincial Key Laboratory for Manufacturing Technology and Application of Pharmaceutical Preparation,Hefei 230012,China;Anhui Provincial Key Laboratory for Chinese Medicinal Formula,Hefei 230012,China)

机构地区：[1]安徽中医药大学药学院,安徽合肥230012 [2]新安医学教育部重点实验室,安徽合肥230012 [3]药物制剂技术与应用安徽省重点实验室,安徽合肥230012 [4]中药复方安徽省重点实验室,安徽合肥230012

出　　处：《中成药》2021年第3期569-574,共6页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金面上项目(81874348);安徽省自然科学基金杰青项目(1908085J29);安徽省重点研究与开发计划(对外科技合作)专项(201904b11020023)。

摘　　要：目的研究桔梗多糖对桔梗皂苷D在体肠吸收的影响。方法大鼠随机分为对照组及桔梗多糖低、中、高剂量组,建立在体肠灌注模型,考察桔梗多糖对桔梗皂苷D吸收速率常数(K_(a))、表观吸收系数(P_(app))、口服药物吸收分数(fa)的影响。灌注后,取下大鼠肠段,免疫荧光染色法检测Claudin⁃1、Occludin、ZO⁃1表达。结果与对照组比较,桔梗多糖组Ka、P_(app)、f_(a)值升高(P<0.05,P<0.01),Claudin⁃1、Occludin、ZO⁃1表达下调(P<0.05,P<0.01),并呈剂量依赖性。结论桔梗多糖可改善桔梗皂苷D在体肠吸收,其机制可能与下调紧密连接蛋白表达有关。AIM To study the effects of Platycodon grandiflorum polysaccharides on in situ intestinal absorption of platycodin D.METHODS Rats were randomly assigned into control group and low⁃dose,medium⁃dose,high⁃dose P.grandiflorum polysaccharides groups,the in situ intestinal perfusion model was established,after which the effects of P.grandiflorum polysaccharides on absorption rate constant(K_(a)),apparent absorption coefficient(P_(app))and absorption fraction of oral medicine(f_(a))of platycodin D were investigated.After the perfusion,the rat intestinal segment was collected,and Claudin⁃1,Occludin and ZO⁃1 expressions were detected by immunofluorescence staining method.RESULTS Compared with the control group,the P.grandiflorum polysaccharides groups demonstrated increased Ka,Papp and f_(a) values(P<0.05,P<0.01),and down⁃regulated Claudin⁃1,Occludin and ZO⁃1 expressions(P<0.05,P<0.01)in a dose⁃dependent manner.CONCLUSION P.grandiflorum polysaccharides can improve the in situ intestinal absorption of platycodin D,whose mechanism may contribute to the down⁃regulation of tight junction protein expressions.

关 键 词：桔梗多糖 桔梗皂苷D 在体肠吸收 免疫荧光染色法 

分 类 号：R969.1[医药卫生—药理学]