UPLC⁃QQQ⁃MS/MS法同时测定风湿安泰片中12种成分  

Simultaneous determination of twelve constituents in Fengshi Antai Tablets by UPLC⁃QQQ⁃MS/MS

作　　者：陈玉华 卢松 CHEN Yu-hua;LU Song(College of Medicine,Hubei Polytechnic University,Huangshi 435000,China;Department of Rehabilitation,Huangshi Hospital of Traditional Chinese Medicine,Huangshi 435003,China)

机构地区：[1]湖北理工学院医学院,湖北黄石435000 [2]黄石市中医医院康复科,湖北黄石435003

出　　处：《中成药》2021年第3期596-600,共5页Chinese Traditional Patent Medicine

摘　　要：目的建立UPLC⁃QQQ⁃MS/MS法同时测定风湿安泰片(马钱子、羌活、麻黄等)中麻黄碱、伪麻黄碱、士的宁、马钱子碱、绿原酸、小檗碱、柚皮苷、人参皂苷Re、人参皂苷Rg1、淫羊藿苷、人参皂苷Rb1、异欧前胡素的含量。方法该药物70%甲醇提取液的分析采用Agilent Zorbax SB⁃C_(18)色谱柱(100 mm×2.1 mm,1.8μm);流动相水(含0􀆰1%乙酸)⁃甲醇,梯度洗脱;体积流量0.2 mL/min;柱温40℃;ESI离子源正离子模式,多反应监测。结果12种成分在各自范围内线性关系良好(r>0.9983),平均加样回收率93.5%~103.2%,RSD 0.9%~1.9%。结论该方法简单灵敏,专属性强,可用于风湿安泰片的质量控制。AIM To establish a UPLC⁃QQQ⁃MS/MS method for the simultaneous content determination of ephedrine,pseudoephedrine,strychnine,brucine,chlorogenic acid,berberine,naringin,ginsenoside Re,ginsenoside Rg1,icariin,ginsenoside Rb1 and isoimperatorin in Fengshi Antai Tablets(Strychni Semen,Notopterygii Rhizoma et Radix,Ephedrae Herba,etc.).METHODS The analysis of 70%methanol extract of this drug was performed on a 40℃thermostatic Agilent Zorbax SB⁃C_(18) column(100 mm×2.1 mm,1.8μm),with the mobile phase comprising of water(containing 0.1%acetic acid)⁃methanol flowing at 0.2 mL/min in a gradient elution manner,and ESI source was adopted in positive mode with multiple⁃reaction monitoring.RESULTS Twelve constituents showed good linear relationships within their own ranges(r>0.9983),whose average recoveries were 93.5%-103.2%with the RSDs of 0.9%-1.9%.CONCLUSION This simple,sensitive and specific method can be used for the quality control of Fengshi Antai Tablets.

关 键 词：风湿安泰片 化学成分 UPLC⁃QQQ⁃MS/MS 

分 类 号：R927.2[医药卫生—药学]