吡嗪酰胺对小鼠肠道微生物的影响  

Effects of Different Concentrations of Pyrazinamide on Intestinal Microorganisms in Mice

作　　者：姜明照 缪志敏 赵莹莹 赖泳 Jiang Mingzhao;Miao Zhimin;Zhao Yingying;Lai Yong(College of Pharmacy,Dali University,Dali,Yunnan 671000,China)

机构地区：[1]大理大学药学院,云南大理671000

出　　处：《大理大学学报》2021年第2期20-24,共5页Journal of Dali University

基　　金：国家自然科学基金项目(81360511)。

摘　　要：目的:探讨吡嗉酰胺对小鼠肠道菌群的影响。方法:设置溶剂对照组、吡嗪酰胺低剂量组和吡嗉酰胺高剂量组,每组10只,采集3组小鼠新鲜粪便进行16SrRNA测序,并利用生物信息学方法分析小鼠肠道菌群多样性。结果:吡嗪酰胺低剂量组、高剂量组和溶剂对照组肠道菌群在门水平上以拟杆菌门和厚壁菌门为优势菌群,其占比依次为47.02.%、51.46%,56.24%、41.65%和76.14%、20.83%。属水平上,吡嗪酰胺低剂量组以拟杆菌属(8.42%)和颤螺旋菌属(1.41%)为主;吡嗪酰胺高剂量组以乳酸杆菌属(2.74%)、Odoribacter(1.20%)和拟杆菌属(1.14%)为主;溶剂对照组以普雷沃菌属(3.54%)和双歧杆菌属(1.81%)为主。相对于溶剂对照组,吡嗉酰胺组的氨基酸、能量和多糖代谢均显著降低(P<0.05)。结论:吡嗉酰胺能够改变肠道菌群结构组成及代谢功能。Objective:To investigate the effects of pyrazinamide on intestinal flora in mice.Methods:A solvent control group,a lowdose pyrazinamide group and a high-dose pyrazinamide group were set up with 10 mice in each group.Three groups of fresh feces were collected for 16 S rRNA sequencing and analyzed by bioinformatics methods to determine the diversity of mouse intestinal flora.Results:At the phylum level,the low-dose group,the high-dose group,and the solvent control group are dominated by Bacteroidetes and Firmicutes,and their dominant flora accounted for 47.02%,51.46%,56.24%,41.65%,76.14%and 20.83%,respectively.At the genus level,the low-dose group is dominated by Bacteroides(8.42%)and Oscillospira(1.41%);the high-dose group is dominated by Lactobacillus(2.74%),Odoribacter(1.20%)and Bacteroides(1.14%);the solvent control group is mainly Prevotella(3.54%)and Bifidobacterium(1.81%).Compared with the solvent control group,the amino acid,energy and polysaccharide metabolism of the pyrazinamide group were significantly reduced(P<0.05).Conclusion:Pyrazinamide can change the composition and metabolic function of intestinal flora.

关 键 词：吡嗪酰胺 肠道菌群 16S rRNA 

分 类 号：R285.5[医药卫生—中药学]