黑毛石斛×鼓槌石斛杂交种组培快繁技术研究  

Tissue Culture and Rapid Propagation Technology in Dendrobium williamsonii×D.chrysotoxum

作　　者：田英男 张天泽 秦晓杰 冯垒 胡淞 师凯辉 黄鑫 韩欣妤 朱永平[1] 和凤美[1,2] TIAN Yingnan;ZHANG Tianze;QIN Xiaojie;FENG Lei;HU Song;SHI Kaihui;HUANG Xin;HAN Xinyu;ZHU Yongping;HE Fengmei(College of Horticulture and Landscape,Yunnan Agricultural University,Kunming,Yunnan 650201,China;Yunnan Provincial Key Laboratory of Orchidaceae Plant Research,Kunming,Yunnan 650201,China;College of Arts and Sciences,Yunnan Normal University,Kunming,Yunnan 650222,China)

机构地区：[1]云南农业大学园林园艺学院,云南昆明650201 [2]云南省高校兰科植物研究重点实验室,云南昆明650201 [3]云南师范大学文理学院城市学院,云南昆明650222

出　　处：《热带作物学报》2021年第2期356-361,共6页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金项目(No.K1400388000)。

摘　　要：本研究以黑毛石斛×鼓槌石斛杂交种蒴果为外植体,通过对种子无菌萌发和原球茎诱导、原球茎增殖及丛生芽诱导、生根壮苗以及移栽驯化研究,建立杂交石斛兰的组培快繁技术体系。结果表明,最适合种子无菌萌发和原球茎诱导的培养基为MS+6-BA 0.5 mg/L+NAA 0.3 mg/L+琼脂6.8 g/L+蔗糖20 g/L+马铃薯50 g/L;原球茎增殖及丛生芽诱导的最佳培养基为1/2 MS+6-BA 1.0 mg/L+NAA 0.2 mg/L+KT 1.0 mg/L+蔗糖25 g/L+琼脂6.8 g/L+碳粉1.0 g/L+马铃薯50 g/L+香蕉50 g/L,继代培养50 d,增殖系数达6.19;最佳生根壮苗培养基为1/2 MS+6-BA 0.2 mg/L+NAA 0.5 mg/L+花宝1号0.5 g/L+蔗糖25 g/L+琼脂6.8 g/L+碳粉1.0 g/L+马铃薯50 g/L+香蕉50 g/L,培养2个月后,平均株高达7.14 cm,平均根数6.17条,平均根长6.46 cm,生根率达100%。体积比为单一松树皮和松树皮∶兰石=1∶1适于移栽,移栽65 d成活率分别达到96.32%和96.71%。In order to establish a rapid propagation for Dendrobium williamsonii×D.chrysotoxum,the immature hybrid seeds were selected as the explants to study the suitable medium for aseptic seed germination,protocorm-like bodies(PLBs)induction,proliferation of protocorm and shoot induction,rooting and growth and domestication by transplanting were also screened.MS+6-BA 0.5 mg/L+NAA 0.3 mg/L+agar 6.8 g/L+sucrose 20 g/L+potato 50 g/L was the best medium for hybrid seeds germination and protocorm induction.1/2 MS+6-BA 1.0 mg/L+NAA 0.2 mg/L+KT 1.0 mg/L+sucrose 25 g/L+agar 6.8 g/L+AC 1.0 g/L+potato 50 g/L+banana 50 g/L was suitable for protocorm proliferation and shoot induction,and the proliferation coefficient reached 6.19 times after 50d.The best rooting and growth medium was 1/2 MS+6-BA 0.2 mg/L+NAA 0.5 mg/L+Hyponex No.10.5 g/L+sucrose 25 g/L+agar 6.8 g/L+AC 1.0 g/L+potato 50 g/L+banana 50 g/L,cultivated for 2 months,root system was long and strong,plant height was 7.14 cm,average number of roots was 6.17,average root length was 6.46 cm,rooting rate was 100%.In the pine bark and mixture of pine bark∶orchid stone=1∶1(V∶V),after cultured for 65 d,the survival percentage was 96.32%and 96.71%,respectively.

关 键 词：黑毛石斛 鼓槌石斛 杂交育种 组织培养 

分 类 号：S567.239[农业科学—中草药栽培]