八味沉香散对缺血再灌注损伤心肌细胞SOD、MDA和凋亡影响的研究  

Effect of Bawei Chenxiang Powder on SOD,MDA and apoptosis of myocardial cells in ischemia-reperfusion injury

作　　者：徐冰[1] 角加 聂波[2] XU Bing;JIAO Jia;NIE Bo(Department of Traditional Chinese Medicine,Beijing Tibetan Hospital,China Tibetology Research Center,Beijing 100029,China;Dongzhimen Hospital,Beijing University of Chinese Medicine/Ministry of Education and Beijing Key Laboratory of Internal Medicine of Traditional Chinese Medicine,Beijing 100700,China)

机构地区：[1]中国藏学研究中心北京藏医院中医科,北京100029 [2]北京中医药大学东直门医院/中医内科学教育部暨北京市重点实验室,北京100700

出　　处：《吉林中医药》2021年第3期376-380,共5页Jilin Journal of Chinese Medicine

基　　金：国家自然青年基金(81704133)。

摘　　要：目的观察八味沉香散对缺血再灌注损伤大鼠心肌细胞H9C2 SOD活力、MDA浓度以及细胞凋亡的影响。方法H9C2细胞随机分为6组:正常组、缺血再灌注组(I/R组)、八味沉香散提取物(水提)高、中、低剂量组(240、120、60μg/mL)、曲美他嗪组(240μg/mL)。以体外缺糖缺氧再复糖复氧建立缺血再灌注损伤模型,WST-1法检测细胞内SOD活力,TBA法检测MDA浓度,Hoechst 33258染色法检测细胞凋亡水平。结果模型组细胞受到缺血再灌注损伤刺激后,细胞内MDA浓度明显增高,SOD活力下降,荧光显微镜下有大量致密或碎块状的浓染细胞核,半定量图像分析平均光密度增高(P<0.01),与正常组比较有显著差异。与模型组比较,八味沉香散提取物中的MDA含量降低(P<0.01),而SOD活力显著增高(P<0.05,P<0.01),荧光显微镜下的致密浓染细胞核明显减少、半定量图像分析平均光密度显著降低(P<0.01)。结论八味沉香散能够减少脂质过氧化物(MDA)生成,提高具有抗氧化能力SOD的活力,抑制细胞凋亡。推测八味沉香散抗缺氧再灌注损伤是通过减少应激状态下活性氧化自由基的形成,从而阻断活性氧化自由基诱发细胞凋亡的直接和间接途径,达到保护心肌细胞的作用。Objective To observe the eff ect of Bawei Chenxiang Powder on SOD activity,MDA concentration and apoptosis of H9C2 myocardial cells in rats with ischemia-reperfusion injury.Methods H9C2 cells were randomly divided into 6 groups:normal group,ischemia-reperfusion group(I/R group),Bawei Chenxiang Powder extract(water extract)high,medium and low dose groups(240,120,60μg/mL),trimetazidine group(240μg/mL).The model of ischemia-reperfusion injury was established by in vitro hypoglycemia,hypoxia and reoxygenation.Intracellular SOD activity was detected by WST-1 method,while MDA concentration was detected by TBA method,and apoptosis was detected by Hoechst 33258 staining.Results After the cells in the model group were stimulated by ischemiareperfusion injury,in the model group,intracellular MDA concentration increased significantly,SOD activity decreased,and a large number of dense or fragmented densely stained cell nuclei were observed under fl uorescence microscope.The average optical density of semi quantitative image analysis increased,which was significantly different from that of the normal group(P<0.01).Compared with the model group,the MDA content in Bawei Chenxiang Powder extract was decreased(P<0.01),while the SOD activity was significantly increased(P<0.05,P<0.01),and densely stained nuclei under the fl uorescence microscope were signifi cantly reduced,and the average optical density of semi quantitative image analysis was significantly decreased(P<0.01).Conclusion Bawei Chenxiang Powder can reduce the production of lipid peroxide(MDA),improve the activity of SOD with antioxidant capacity,and inhibit cell apoptosis.It is speculated that Bawei Chenxiang Powder can protect myocardial cells by reducing the formation of active oxidative free radicals under stress,thus blocking the direct and indirect pathways of apoptosis induced by active oxidative free radicals.

关 键 词：八味沉香散 MDA SOD 凋亡 缺氧再灌注损伤 

分 类 号：R285.5[医药卫生—中药学]