Micro-computed tomography utility for estimation of intraparenchymal spinal cord cystic lesions in small animals  

作　　者：Mahmoud Farrag Dipak D.Pukale Nic D.Leipzig 

机构地区：[1]Integrated Bioscience Program,The University of Akron,Akron,OH,USA [2]Department of Chemical,Biomolecular,and Corrosion Engineering,The University of Akron,Akron,OH,USA [3]Department of Chemical,Biomolecular,and Corrosion Engineering,Integrated Bioscience Program,The University of Akron,Akron,OH,USA

出　　处：《Neural Regeneration Research》2021年第11期2293-2298,共6页中国神经再生研究（英文版）

基　　金：This study was financially supported by Conquer Chiari.

摘　　要：Precise assessment of spinal cord cystic lesions is crucial to formulate effective therapeutic strategies,yet histological assessment of the lesion remains the primary method despite numerous studies showing inconsistent results regarding estimation of lesion size via histology.On the other hand,despite numerous advances in micro-computed tomography(micro-CT)imaging and analysis that have allowed precise measurements of lesion size,there is not enough published data on its application to estimate intraspinal lesion size in laboratory animal models.This work attempts to show that micro-CT can be valuable for spinal cord injury research by demonstrating accurate estimation of syrinx size and compares between micro-CT and traditional histological analysis.We used a post-traumatic syringomyelia rat model to compare micro-CT analysis to conventional histological analysis.The study showed that micro-CT can detect lesions within the spinal cord very similar to histology.Importantly,micro-CT appears to provide more accurate estimates of the lesions with more measures(e.g.,surface area),can detect compounds within the cord,and can be done with the tissue of interest(spinal cord)intact.In summary,the experimental work presented here provides one of the first investigations of the use of micro-CT for estimating the size of intraparenchymal cysts and detecting materials within the spinal cord.All animal procedures were approved by the University of Akron Institutional Animal Care and Use Committee(IACUC)(protocol#LRE 16-05-09 approved on May 14,2016).

关 键 词：cyst size histology intraparenchymal cysts lesion size estimate micro-CT post-traumatic syringomyelia spinal cord imaging spinal cord injury spinal cystic lesion SYRINX 

分 类 号：R445[医药卫生—影像医学与核医学] R364[医药卫生—诊断学]