大骨节病和骨性关节炎软骨细胞外基质的降解差异  

Difference in extracellular matrix loss between KBD and osteoarthritis

作　　者：武世勋[1,2] 吴翠艳[2] 郭雄[2] WU Shixun;WU Cuiyan;GUO Xiong(Department of Orthopedics Surgery,The Third Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710068;Institute of Endemic Diseases of School of Public Health,Xi’an Jiaotong University Health Science Center,National Health Commission Key Laboratory of Trace Elements and Endemic Diseases,Collaborative Innovation Center of Endemic Diseases and Health Promotion in Silk Road Region,Xi’an 710061,China)

机构地区：[1]西安交通大学第三附属医院,陕西西安710068 [2]西安交通大学医学部,国家卫健委微量元素与地方病重点实验室,陕西省丝路区域地方病与健康促进协同创新中心,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2021年第2期306-310,316,共6页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.81620108026;No.81472924);陕西省人民医院科技发展孵化基金(No.2020YXM-12)。

摘　　要：目的研究大骨节病和骨关节炎软骨组织细胞外基质的主要丢失成分。方法收集临床手术遗弃的关节软骨标本,制备石蜡切片,采用HE染色、甲苯胺蓝染色、番红花素染色半定量分析大骨节病及骨关节炎软骨组织中细胞外基质的含量差异。结果正常组(NC组)HE染色面积百分比(83.65±8.38)%明显高于原发性骨关节炎组(OA组)(57.90±21.88)%和大骨节病组(KBD组)(43.67±23.91)%,OA组的染色面积百分比高于KBD组(P=0.034);NC组番红花精O染色面积百分比(75.66±12.54)%明显高于OA组(53.81±10.48)%和KBD组(62.07±14.66)%,KBD组明显高于OA组(P=0.011);NC组、OA组和KBD组的甲苯胺蓝染色面积百分比差异无统计学意义(P>0.05)。结论 KBD软骨组织的细胞外基质总量丢失比OA软骨多,OA关节软骨中蛋白多糖的丢失比KBD软骨更严重,提示KBD软骨细胞外基质丢失可能以Ⅱ型胶原蛋白为主。Objective To study the major missing components of extracellular matrix in cartilage from Kashin-Beck disease(KBD) and osteoarthritis(OA). Methods The articular cartilage specimens discarded in clinical surgery were collected;paraffin sections were prepared;and HE staining, toluidine blue, and crocusin staining were used to semi-quantitatively analyze the differences in the content of extracellular matrix in cartilage from KBD and OA. Results The percentage of HE staining area in the normal group(NC group)(83.65±8.38)% was significantly higher than that in the primary osteoarthropathy group(OA group)(57.90±21.88)% and the KBD group(KBD group)(43.67±23.91)%. The stained area percentage of the OA group was higher than that of the KBD group(P=0.034). In the NC group(75.66±12.54)% of the saffron essence O stained area percentage was significantly higher than that of the OA group(53.81±10.48)% and the KBD group(62.07±14.66)%;the KBD group was higher than the OA group(P=0.011). No statistically significant difference in area percentage was found among the NC group, OA group and KBD group stained with toluidine blue(P>0.05). Conclusion The total loss of extracellular matrix of KBD cartilage tissue is more than that of OA cartilage. The loss of proteoglycan in OA articular cartilage is more serious than that in KBD cartilage, suggesting that the lost extracellular matrix of KBD cartilage tissue is mainly type 2 collagen.

关 键 词：大骨节病 软骨细胞外基质 骨关节炎 透明质酸 硫酸软骨素 

分 类 号：R681.3[医药卫生—骨科学]