分泌卷曲相关蛋白4肝脏高表达转基因小鼠的制作及鉴定分析  

Establishment and identification of secreted frizzled-related protein 4 transgenic mice and analysis of their biological properties

作　　者：关华 郑慧媛[3] 刘恩岐[4] 徐礼鲜 余琦 GUAN Hua;ZHEN Huiyuan;LIU Enqi;XU Lixian;YU Qi(Key Laboratory of Ischemic Cardiovascular Disease in Shaanxi&Institute of Basic and Translational Medicine of Xi’an Medical College,Xi’an 710021;Department of Anesthesiology,The Fourth Military Medical University Stomatological Hospital,Xi’an 710032;Department of Histology and Embryology,Xi’an Medical College,Xi’an 710021;Laboratory for Lipid Metabolism and Atherosclerosis,Xi’an Jiaotong University Cardiovascular Research Center,Xi’an 710061,China)

机构地区：[1]西安医学院基础与转化医学研究所陕西省缺血性心血管疾病重点实验室,陕西西安710021 [2]空军军医大学口腔医院麻醉科,陕西西安710032 [3]西安医学院组织学与胚胎学教研室,陕西西安710021 [4]西安交通大学心血管研究中心脂代谢与动脉粥样硬化研究室,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2021年第2期328-332,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.81900399,81400328,81773795);陕西省教育厅重点科研计划项目(No.19JS060);陕西省中医药局科技计划项目(No.2019-ZZ-JC034);西安医学院博士科研启动经费(No.2018DOC12)。

摘　　要：目的制作肝脏特异性高表达分泌卷曲相关蛋白4(SFRP4)的转基因小鼠,并对其生物学特性进行分析。方法利用显微注射方法制作SFRP4转基因小鼠,采用Western blotting和qRT-PCR技术鉴定模型小鼠中SFRP4转基因的表达水平。结果 PCR结果显示,成功获得SFRP4转基因小鼠,SFRP4转入基因主要在肝脏中表达,肝脏组织中SFRP4基因和蛋白表达水平与野生型同窝对照组相比明显升高。结论成功建立了高表达SFRP4的转基因小鼠,为研究SFRP4基因功能和参与代谢性疾病的机制提供了良好的动物模型。Objective To generate secreted frizzled-related protein 4(SFRP4) transgenic mice and analyze their biological properties. Methods We generated SFRP4 transgenic mice by DNA microinjection and detected the expression of SFRP4 by qRT-PCR and Western blotting. Results SFRP4 transgenic mice were successfully generated by DNA microinjection. The expression of SFRP4 was dramatically increased in transgenic mice liver compared with that of wide type. Conclusion The transgenic mice model of SFRP4 was successfully established by DNA microinjection. It provides a good model for studying the function of SFRP4 and the mechanism of participating in metabolic diseases.

关 键 词：分泌卷曲相关蛋白4 转基因小鼠 血浆 肝脏 

分 类 号：Q95-33[生物学—动物学]