基于AFLP分子标记的广藿香遗传多样性分析  

Genetic Diversity Analysis of Pogostemon cablin Based on AFLP Markers

作　　者：刘晓莹 李嘉惠 杨钰婷 严寒静 张宏意 何梦玲 LIU Xiao-ying;LI Jia-hui;YANG Yu-ting;YAN Han-jing;ZHANG Hong-yi;HE Meng-ling(School of Traditional Chinese Medicine(TCM),Key Laboratory of State Administration of TCM for Production&Development of Cantonese Medicinal Materials.Comprehensive Experimental Station of Guangzhou,Chinese Materia Medica,China Agriculure Research System(CARS-21-16),Guangdong Pharmaceutical University,Guangzhou 510006,China)

机构地区：[1]广东药科大学中药学院,国家中医药管理局岭南药材生产与开发重点研究室,中药材国家现代农业产业技术体系广州综合试验站(CARS-21-16),广州510006

出　　处：《中国实验方剂学杂志》2021年第4期152-158,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广东省科技项目(2015A030302084,2017A030303082,2017A030303081);国家自然科学基金项目(81773829);广东省重点领域研发计划项目(2020B020221002);广东省中管局项目(20163010)。

摘　　要：目的:采用扩增片段长度多态性(AFLP)分子标记方法研究广藿香的种质遗传多样性。方法:采用12对引物对14个品种共212个样本进行AFLP分析;利用POPGENE 32,Arlequinver 3.5,MEGA7,NTSYSpc 2.10e等生物学分析软件进行多态性参数计算、主坐标分析以及聚类分析。结果:12对引物共扩增出2 238个位点,其中多态性位点有2 226个,多态位点百分率为99.38%;在种群间,有效等位基因数(Ne)为1.365 6±0.066 3,Nei’s基因多样性指数(H)为0.220 7±0.036 4,Shannon多态性信息指数(I)为0.343 7±0.050 2;在种群内,Ne为1.118 5±0.038 7,H为0.071 3±0.023 0,I为0.109 4±0.035 0;分子方差学分析(AMOVA)表明广藿香的总变异有71.57%来源于种群间,28.43%来源于种群内;聚类分析可将14个种群分为4大类。结论:AFLP分子标记结果显示,广藿香种间具有比较丰富的遗传多样性,种内遗传多样性相对较小,种间遗传分化显著,可为广藿香后续的优良种质筛选等研究提供参考依据。Objective:This paper aims to study the genetic diversity of Pogostemon cablin by amplified fragment length polymorphism(AFLP)markers. Method: The 12 pairs of primers were used for AFLP analysis of 212 samples from 14 varieties,and biological analysis software such as POPGENE 32,Arlequinver3.5,MEGA 7 and NTSYSpc 2.10 e were used for polymorphism parameter calculation,principal coordinate analysis and cluster analysis. Result:A total of 2 238 loci were amplified by 12 pairs of primers. 2 226 of them were polymorphic loci,accounting for 99.38%. At the inter-population level,the values of effective alleles(Ne),Nei’s gene diversity index(H),Shannon polymorphic information index(I)were 1.365 6±0.066 3,0.220 7±0.036 4,and 0.343 7±0.050 2,respectively;and 1.118 5±0.038 7,0.071 3±0.023 0,0.109 4±0.035 0,respectively at the intra-population level. Analysis of molecular variance(AMOVA)showed that 71.57% of the total variation of P. cablin was of inter-population nature,and 28.43% was of intra-population nature. The 14 populations could be divided into four groups by cluster analysis. Conclusion:The results of AFLP molecular markers showed that abundant genetic diversity was present at inter-population level of P. cablin,however,relatively low at intrapopulation level;the genetic differentiation at the inter-population level was significant,which could provide a reference for the subsequent study of good germplasm selection of P. cablin.

关 键 词：广藿香 扩增片段长度多态性 种质资源 遗传多样性 

分 类 号：R284.2[医药卫生—中药学] R289[医药卫生—中医学]