大豆胞囊线虫胁迫下GmCHS基因表达及异黄酮含量变化分析  

Expression Analysis of GmCHS Gene and Isoflavone Content Changes Under Soybean Cyst Nematode Stress

作　　者：王爽 张顺斌 王旭东 于佰双[2] 陈井生[3] 王惠[1] 段玉玺[4] WANG Shuang;ZHANG Shun-bin;WANG Xu-dong;YU Bai-shuang;CHEN Jing-sheng;WANG Hui;DUAN Yu-xi(College of Bioscience and Biotechnology,Shenyang Agricultural University,Shenyang 110866,China;Heilongjiang Academy of Agricultural Sciences,Harbin 150086,C hina;Daqing Branch of Heilongjiang Academy of Agricultural Sciences,Daqing 163316,C hina;College of Plant Protection,Shenyang Agricultural University,Shenyang 110866,China)

机构地区：[1]沈阳农业大学生物科学技术学院,辽宁沈阳110866 [2]黑龙江省农业科学院,黑龙江哈尔滨150086 [3]黑龙江省农业科学院大庆分院,黑龙江大庆163316 [4]沈阳农业大学植物保护学院,辽宁沈阳110866

出　　处：《大豆科学》2021年第1期21-27,共7页Soybean Science

基　　金：国家自然科学基金重点项目(31330063)。

摘　　要：异黄酮作为植保素,在生物因素、非生物因素的胁迫下,都发挥着重要抗逆作用,而查尔酮合成酶(CHS)是异黄酮形成途径中的关键位点,为研究GmCHS在大豆胞囊线虫胁迫下的响应表达,以便进一步揭示大豆抗大豆胞囊线虫的分子机理,本研究选取抗病品种灰皮支黑豆和感病品种Williams 82,在大豆胞囊线虫3号生理小种胁迫后的不同时期检测CHS5、CHS7、CHS8、CHS9基因的表达,并测定其下游产物异黄酮成分中的大豆苷元和黄豆黄素的含量。结果显示:感病品种中CHS5、CHS7、CHS8、CHS9基因的相对表达量变化程度较抗病品种小。胁迫5 d时,抗病品种中4个查尔酮合成酶基因均明显上调表达,与实验室前期大豆转录组测序中CHS7、CHS8均上调表达结果一致。进一步对两个品种主要异黄酮成分含量的检测结果表明,大豆苷元相较于黄豆黄素在大豆胞囊线虫胁迫下响应更加敏感,其中大豆苷元在Williams 82无响应,而在灰皮支黑豆接种5,10,15 d均出现了显著差异,可见异黄酮中的大豆苷元很可能是响应大豆胞囊线虫胁迫的重要成分之一。因此,初步认为CHS基因在抗大豆胞囊线虫的抗性机制中具有重要作用。As phytoalexin, under the stress of biotic and abiotic factors, isoflavones play an important role in resisting stress. Chalcone synthase(CHS) is a key site in isoflavone formation pathway, CHS response expression under soybean cyst nematode stress, it is beneficial to further reveal the molecular mechanism of soybean resistance to soybean cyst nematode. In this study, we selected disease-resistant varieties of Huipizhi Heidou and susceptible varieties of Williams 82 as the research objects. The expressions of CHS5, CHS7, CHS8 and CHS9 were detected at different periods after the treatment of soybean cyst nematode race 3, and the content of daidzein and glycintein in the downstream product isoflavones were determined. According to the results, the relative expression of CHS5, CHS7, CHS8 and CHS9 genes in susceptible varieties changed less than resistant varieties. At 5 day of stress, the 4 CHS genes in the disease-resistant cultivars were all significantly up-regulated, which was consistent with the up-regulated expression results of CHS7 and CHS8 of soybean transcriptome sequencing. Under the stress of SCN3, combined with soybean transcriptome sequencing analysis results showed that CHS7 and CHS8 up-regulated expression. Further detection of the content of the main isoflavone components of the 2 varieties showed that daidzein was more sensitive to the response of soybean cyst nematode than glycintein. Among them, daidzein did not respond to Williams 82, however, there were significant differences in the 5, 10 and 15 days of the inoculation of the Huipizhi Heidou. It could be seen that the daidzein in isoflavones was likely to be one of the important components in response to soybean cyst nematode stress. Therefore, it was preliminarily believed that the CHS gene plays an important role in the resistance mechanism of soybean cyst nematode.

关 键 词：大豆 大豆胞囊线虫 查尔酮合成酶 差异表达基因 异黄酮 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]