地椒中1个新的二氢黄酮化合物  

A novel flavanone from Thymus przewalskii

作　　者：常小强 马悦[1,2] 孙鹏 高鹏[1,2] 赵一帆[1,2] 谷丽维 张东[1,2] 杨岚 田纪祥[1] CHANG Xiao-qiang;MA Yue;SUN Peng;GAO Peng;ZHAO Yi-fan;GU Li-wei;ZHANG Dong;YANG Lan;TIAN Ji-xiang(Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Artemisinin Research Center,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]中国中医科学院青蒿素研究中心,北京100700

出　　处：《中国中药杂志》2021年第1期125-129,共5页China Journal of Chinese Materia Medica

基　　金：中央本级重大增减支项目(2060302);国家“重大新药创制”科技重大专项(2019ZX09201005)。

摘　　要：研究地椒的化学成分,利用硅胶,ODS,Sephadex LH-20和半制备HPLC等色谱方法分离纯化,结合其MS,NMR,UV,IR和CD等现代分析技术鉴定化合物的结构。从地椒的乙醇提取物乙酸乙酯萃取部位分离并鉴定了4个二氢黄酮化合物,分别为(2S)-5,6-dihydroxy-7,8,4'-trimethoxyflavanone(1),5,4'-dihydroxy-6,7-dimethoxyflavanone(2),(2S)-5,4'-dihydroxy-7,8-dimethoxyflavanone(3),樱花素(4)。其中,化合物1为新化合物,并通过CD谱确定其构型,化合物3为首次分离得到的天然产物,并通过CD谱确定其构型,化合物2为首次从百里香属分离得到,化合物4为首次从地椒中分离得到。体外细胞毒性试验结果表明,化合物1~4对人类肺癌细胞A549具有微弱的细胞毒性,IC_(50)为74.5~135.6μmol·L^(-1)。This study was to investigate the chemical constituents from the aerial parts of Thymus przewalskii.The chemical constituents were separated and purified by column chromatography on silica gel,ODS,Sephadex LH-20 and semi-prepared HPLC,and their structures were determined by physicochemical properties and spectroscopic data.Four flavanones were isolated from the ethanol extract of the aerial parts of T.przewalskii,and identified as(2S)-5,6-dihydroxy-7,8,4'-trimethoxyflavanone(1),5,4'-dihydroxy-6,7-dimethoxyflavanone(2),(2S)-5,4'-dihydroxy-7,8-dimethoxyflavanone(3),sakuranetin(4),respectively.Compound 1 was a new compound and its configuration was determined by CD spectrum,compound 3 was natural product which was isolated for the first time and their configurations were determined by CD spectra.Compound 2 was isolated from the genus Thymus for the first time and compound 4 was isolated from T.przewalskii for the first time.Furthermore,cytotoxicity test was assayed for the four flavanones.They exhibited weak cytotoxicity against human lung cancer cells(A549),with the IC_(50) from 74.5 to 135.6μmol·L^(-1).

关 键 词：地椒 化学成分 二氢黄酮 细胞毒性 

分 类 号：R284.1[医药卫生—中药学]