意大利蜜蜂DHPR基因的生物信息学分析  

Bioinformatical analysis for dihydropterin reductase(DHPR)of Apis mellifera mellifera

作　　者：舒蕊 刘健[1] 赵萍[1] 刘震[1] 代君君[1] Shu Rui;Liu Jian;Zhao Ping;Liu Zhen;Dai Junjun(Institute of Sericulture,Anhui Academy of Agricultural Sciences,Hefei 230061,China)

机构地区：[1]安徽省农业科学院蚕桑研究所,合肥230061

出　　处：《中国蜂业》2021年第1期61-64,共4页Apiculture of China

基　　金：安徽省农业科学院蚕桑研究所春蚕基金项目“蜜蜂Dhpr基因的表达模式与重组蛋白酶活性质的研究”(SSF2019-01);安徽省农业科学院“经济昆虫资源多功能利用”科技创新团队(2020YL047)。

摘　　要：四氢生物蝶呤是芳香族氨基酸羟化酶及一氧化氮合酶的辅酶,具有抗氧化和清除活性氮氧化物的功能,二氢蝶呤还原酶是四氢生物蝶呤合成代谢途径中的关键酶。本研究对意大利蜜蜂二氢蝶呤还原酶编码蛋白进行了预测分析,并构建了系统进化树。结果显示,意蜂二氢蝶呤还原酶基因编码一个714bp的开放阅读框,编码237个氨基酸,该预测蛋白的分子量约为26kD,等电点为8.90,二级结构主要由8个α-螺旋区和8个β-折叠区构成,系统进化树分析表明,西方蜜蜂DHPR与大蜜蜂和小蜜蜂的DHPR亲缘关系较近,与传统的生物学分类结果相符。实验结果可为进一步开展意蜂二氢蝶呤还原酶的功能研究提供信息参考。Tetrahydrobiopterin is a coenzyme of aromatic amino acid hydroxylase and nitric oxide synthase.It has the function of antioxidant and scavenging active nitrogen oxides.Dihydropterin reductase(DHPR)is the key enzyme in the synthesis and metabolism of tetrahydrobiopterin.In this study,we predicted and analyzed the proteins encoded by dihydropterin reductase of Apis mellifera and constructed a phylogenetic tree.The results showed that dihydropterin reductase gene encoded a 714bp open reading frame(ORF),encoding 237 amino acids.The molecular weight of the predicted protein was about 26kD,the isoelectric point was 8.90,and the secondary structure was mainly composed of 8α-helix regions and 8β-fold regions.Phylogenetic tree analysis showed that DHPR of Apis mellifera was closely related to DHPR of Apis dorsata and Apis fl orea,which was consistent with the results of traditional biological classifi cation.These experiment results would provide information references for further function research of dihydropterin reductase in Apis mellifera.

关 键 词：意大利蜜蜂 二氢蝶呤还原酶 四氢生物蝶呤 生物信息学 

分 类 号：S891[农业科学—特种经济动物饲养]