抗纤灵对慢性肾功能衰竭模型Smad2-KO小鼠心脏CoⅠ、CoⅢ、AngⅡ、TNF-α、IL-6的影响  

Influence of Kangxianling on the Expressions of CoⅠ, CoⅢ, AngⅡ, TNF-α and IL-6in the Heart of Smad2-KO Mice of CRF Model

作　　者：夏嘉[1] 王颖 刘铭洁 高雅婵 陈文浩 何立群[4] XIA Jia;WANG Ying;LIU Mingjie;GAO Yachan;CHEN Wenhao;HE Liqun(Shanghai City Hospital of Traditional Chinese Medicine,Shanghai 200071,China;Shanghai TCM-Integrated Hospital Affiliated to Shanghai University of TCM;The Affiliated Hospital to Guizhou University of Traditional Chinese Medicine;Shuguang Hospital Affiliated to Shanghai University of TCM)

机构地区：[1]上海中医药大学附属市中医医院,上海200071 [2]上海中医药大学附属中西医结合医院 [3]贵阳中医学院第一附属医院 [4]上海中医药大学附属曙光医院

出　　处：《西部中医药》2021年第2期13-17,共5页Western Journal of Traditional Chinese Medicine

基　　金：上海市卫生健康委员会科研课题(201940327);上海市名老中医学术经验研究工作室资助项目(SHGZS-2017027)。

摘　　要：目的:观察抗纤灵对慢性肾功能衰竭(chronic renal failure,CRF)模型Smad2-KO小鼠心脏Ⅰ型胶原(collagen I,CoI)、Ⅲ型胶原(collagenⅢ,CoⅢ)、血管紧张素Ⅱ(AngiotensinⅡ,AngⅡ)、肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)、白细胞介素6(Interleukin-6,IL-6)水平的影响,探讨抗纤灵治疗CRF的作用机制。方法:将18只假手术Smad2-KO小鼠随机分为假手术组(蒸馏水0.5 mL,灌胃)、抗纤灵假手术组(抗纤灵复方20 g/kg,0.5 mL灌胃)、缬沙坦假手术组(缬沙坦20 mg/kg,0.5 mL灌胃),每组6只。将24只CRF模型Smad2-KO小鼠随机分为模型组(蒸馏水0.5 mL,灌胃)、抗纤灵模型组(抗纤灵复方20 g/kg,0.5 mL灌胃)、缬沙坦模型组(缬沙坦20 mg/kg,0.5 mL灌胃),每组8只。灌胃8周后取材,Elisa法检测心脏AngⅡ的水平,Western blot法测心脏CoI、CoⅢ、TNF-α、IL-6的相对表达。结果:与假手术组比较,模型组的AngⅡ、CoⅠ、CoⅢ、TNF-α、IL-6均显著增高(P<0.01),缬沙坦假手术组的AngⅡ下降(P<0.05)。与模型组比较,抗纤灵模型组的AngⅡ有改善(P<0.05),CoI、CoⅢ、TNF-α、IL-6有显著改善(P<0.01)。缬沙坦模型组的CoI、CoⅢ、AngⅡ、TNF-α、IL-6均明显改善(P<0.01)。抗纤灵模型组与缬沙坦模型组组间比较,缬沙坦在改善AngⅡ方面优于抗纤灵组(P<0.05),其余指标两组间比较差异无统计学意义(P>0.05)。结论:抗纤灵可改善CRF模型Smad2-KO小鼠心脏病变的作用机制可能与降低炎症因子的表达、调节RAS和减少心脏胶原的过度沉积有关。Objective: To observe the influence of Kangxianling on the levels of CoⅠ, CoⅢ, AngⅡ, TNF-αand IL-6 in the heart of Smad2-KO mice of CRF model, and to explore its mechanism of treating CRF. Methods:Eighteen Smad2-KO mice were randomized into sham operation group(distilled water, 0.5 mL, intragastric administration), Kangxianling sham operation group(Kangxianling compound, 20 g/kg, 0.5 mL, intragastric administration) and Valsartan sham operation group(Valsartan, 20 mg/kg, 0.5 mL, intragastric administration), six mice each group. 24 Smad2-KO mice of CRF model were allocated to the model group(distilled water, 0.5 mL,intragastric administration), Kangxianling model group(Kangxianling compound, 20 g/kg, 0.5 mL, intragastric administration) and Valsartan model group(Valsartan, 20 mg/kg, 0.5 mL, intragastric administration), eight mice each group. After drenched for eight weeks, Elisa method was used to detect the levels of cardiac AngⅡ, Western blot method to detect the relative expressions of CoⅠ, CoⅢ, TNF-α and IL-6 in the mouse heart. Results:Compared with sham operation group, the expressions of AngⅡ, CoⅠ, CoⅢ, TNF-α and IL-6 increased remarkably in the model group(P<0.01), the levels of AngⅡ lowered in Valsartan sham operation group(P<0.05). Compared with the model group, the levels of AngⅡ of Kangxianling model group were improved(P<0.05), the expressions of CoⅠ, CoⅢ, TNF-α and IL-6 were improved notably(P<0.01). The expressions of CoⅠ, CoⅢ, AngⅡ, TNF-α and IL-6 were improved notably in Valsartan model group(P<0.01). When Kangxianling model group was compared with Valsartan model group, Valsartan was better than Kangxianling in improving AngⅡ(P<0.05), there was no significant difference between two groups in other indexes(P>0.05). Conclusion: The mechanism of Kangxianling improving cardiac abnormalities of Smad2-KO mice of CRF model might be related to lowering the expression of inflammatory factors, regulating RAS and reducing excessive deposition of cardiac collagen.

关 键 词：抗纤灵 Smad2基因敲除 慢性肾衰 心脏 动物实验 

分 类 号：R285.5[医药卫生—中药学]