生、熟大黄对大承气汤泻下作用的比较研究  

Comparative Study on the Influence of Raw and Prepared Rhubarb on Laxative Effects of Dachengqi Decoction

作　　者：曾海生[1] 周歧骥 覃洪含[2] 郭小葆[1] 林瑶[2] 何思陆[1] ZENG Haisheng;ZHOU Qiji;QIN Honghan;GUO Xiaobao;LIN Yao;HE Silu(Affiliated Hospital of Youjiang Medical University for Nationalites,Baise533000,China;Youjiang Medical University for Nationalites)

机构地区：[1]右江民族医学院附属医院,广西百色533000 [2]右江民族医学院

出　　处：《西部中医药》2021年第2期18-21,共4页Western Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(81360524);广西自然科学基金(2016GXNSFAA380148);广西中药药效研究重点实验室(17-259-200);右江民族医学院校级科研课题(yy2018ky018);广西壮族自治区食品药品监督管理局课题(YTT17003);国家中医药管理局科研三级实验室中(壮)药化学与质量分析实验室[国中医药发2009(21)号]。

摘　　要：目的:研究生、熟大黄对大承气汤泻下作用的影响。方法:将72只小鼠随机分为空白对照组、阳性对照组及生、熟大黄大承气汤低(6 g/kg)、高(10 g/kg)剂量组,共6组,每组12只。分别用代谢笼积分法、炭末推进作用及Na^(+)-K^(+)-ATPasc活性实验,比较各组泻下作用、炭末推进率及Na^(+)-K^(+)-ATPasc活性的差异。结果:生大黄大承气汤组、熟大黄大承气汤组与空白对照组及阳性对照组比较,其对正常小鼠泻下作用、炭末推进作用、抑制Na^(+)-K^(+)-ATPase活性的作用差异均有统计学意义(P<0.05),且生大黄大承气汤各组与熟大黄同剂量组比较泻下作用更显著(P<0.05),并与给药剂量成正相关。结论:生、熟大黄对大承气汤泻下作用的影响差异有统计学意义,这可能与君药生、熟大黄的主要化学成分含量变化引起大承气汤复方中蒽醌类化合物的溶出量变化有关。Objective:To study the impacts of raw and prepared rhubarb on laxative effects of Dachengqi decoction.Methods:All 72 mice were randomized into blank control group,positive control group,low(6g/kg)and high(10g/kg)doses groups of raw and prepared rhubarb Dachengqi decoction,six groups altogether,12 mice each group.To compare the difference in laxative effects,advance rate of carbon powder and Na^(+)-K^(+)-ATPasc activity in different groups using metabolic cage integral method,carbon powder propulsion and Na^(+)-K^(+)-ATPasc activity experiment respectively.Results:When raw rhubarb Dachengqi decoction group and prepared rhubarb Dadnenggi decoction group were compared with blank control group and positive control group,the differences existed in their effects on laxative effects,advance rate of carbon powder and inhibiting Na^(+)-K^(+)-ATPasc activity(P<0.05),laxative effects were more notable when the groups of raw rhubarb Dachengqi decoction were compared with equivalent dose groups of prepared rhubarb(P<0.05),and it was positively related to medication dose.Conclusion:The impacts of raw and prepared rhubarb are different on laxative effects of Dachengqi decoction,and this might be related to the changes in the dissolution of anthraqui-nones of Dachengqi decoction induced by the changes of main chemical ingredients in monarch raw and prepared rhubarb.

关 键 词：大承气汤 大黄 泻下作用 动物实验 

分 类 号：R285.5[医药卫生—中药学]