《中华人民共和国药典》大黄总蒽醌含量测定方法的改进  

Improvement of determination method of total anthraquinone in Rhei Radix et Rhizoma in Pharmacopoeia of the People’s Republic of China

作　　者：卞理[1] 窦志华[1] 王丹丹[1] 陆清溪 Bian Li;Dou Zhihua;Wang Dandan;Lu Qingxi(Department of Pharmacy,the Third Affiliated Hospital of Nantong University,Nantong 226006,China)

机构地区：[1]江苏省南通大学附属南通第三医院药学部,226006

出　　处：《国际中医中药杂志》2021年第2期156-160,共5页International Journal of Traditional Chinese Medicine

基　　金：江苏省第四期“333工程”资助科研项目(BRA2013090);江苏省中医药局科技项目(LZ13168);江苏省南通市应用研究计划项目(BK2013013);南通市卫计委青年科研基金项目(WQ2016018)。

摘　　要：目的对《中华人民共和国药典》中大黄药材含量测定项下总蒽醌测定方法进行改进,并与药典方法进行对比分析,确定改进方法的可行性。方法将大黄总蒽醌提取方法中的双相水解改为单相水解,并采用HPLC法测定大黄药材中总蒽醌含量。色谱条件:色谱柱为Symmetry C18(4.6 mm×250 mm,5μm);流动相为甲醇-0.1%磷酸水(85∶15);流速1 ml/min;柱温30℃;检测波长254 nm。结果芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚分别在0.0033~0.3320µg、0.0069~0.6680µg、0.0023~0.2320µg、0.0104~1.0400µg、0.0084~0.8360µg范围内与峰面积呈良好的线性关系;精密度、稳定性、重复性RSD均小于2%;芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚的加样回收率分别为101.50%、99.30%、99.62%、101.57%、103.11%,RSD均小于2%。结论改进后的方法操作简便,检测结果准确可靠,重复性好,可用于大黄药材的质量控制。Objective To improve the determination method for the total anthraquinone of the Rhei Radix et Rhizoma in the Pharmacopoeia of the People’s Republic of China,and compare this method with the method in the pharmacopoeia to determine the feasibility of such method.Methods By changing the determination of total anthraquinone from biphasic hydrolysis to monophase hydrolysis,the method included in the pharmacopoeia was improved to determine the total anthraquinone content in Rhei Radix et Rhizoma.Chromatographic conditions were Symmetry C18(4.6 mm×250 mm,5μm)chromatographic column;the mobile phase is methanol-0.1%phosphoric acid water(85:15);the flow rate was 1 ml/min;the column temperature is 30℃;the detection wavelength is 254 nm.Results The concentrations of aloe-emodin,rhein,emodin,chrysophanol,physcion in the range of 0.0033-0.3320µg,0.0069-0.6680µg,0.0023-0.2320µg,0.0104-1.0400µg,0.0084-0.8360µg have good linear relationship with the peak area;RSDs of precision,stability and repeatability were less than 2%;the recovery rates of aloe-emodin,rhein,emodin,chrysophanol and physcion were 101.50%,99.30%,99.62%,101.57%,and 103.11%,and the RSDs were less than 2%.Conclusion The improvement method is simple,accurate,reliable and reproducible,which could be used for the quality control of Rhei Radix et Rhizoma.

关 键 词：大黄 蒽醌类 中药药典 含量测定(中药) 

分 类 号：R284.1[医药卫生—中药学]