向海野生翠雀高效无菌苗体系的建立  

Establishment of highly efficient aseptic system of Platycodon grandiflorum (Jacq.) A. DC. in Xianghai

作　　者：鲁娜[1] 冯思彤 王宇达 张胜男 丛建民[1] 高金秋[1] 吴丹[2] LU Na;FENG Sitong;WANG Yuda;ZHANG Shengnan;CONG Jianmin;GAO Jinqiu;WU Dan(Baicheng Normal University,Baicheng 137000,China;Jilin Center for Animal Disease Prevention and Control,Changchun 130062,China)

机构地区：[1]白城师范学院,吉林白城137000 [2]吉林省动物疫病预防控制中心,长春130062

出　　处：《黑龙江畜牧兽医》2021年第1期113-117,126,164,共7页Heilongjiang Animal Science And veterinary Medicine

基　　金：吉林省教育厅“十三五”科学技术项目(JJKH20170002KJ);吉林省高等学校大学生创新创业训练项目(2017002);白城师范学院2015年度校级重点项目([2015]A1号)。

摘　　要：为确立向海国家自然保护区野生翠雀高效无菌苗获得的最佳体系,试验采用NaClO消毒法对影响无菌苗获得的NaClO消毒浓度、消毒时间和培养温度进行筛选,采用激素浸种和热激解休眠法对影响种子萌发的激素种类、浸种浓度及热激处理温度、时间进行筛选。结果表明:无菌水浸种、55℃热激10 min、1%NaClO消毒20 min、15℃光照培养为野生翠雀无菌苗获得的最佳体系,无菌苗获得率为90.00%。说明野生翠雀种子解休眠同消毒程序配合处理能够有效促进种子萌发并获得高效无菌苗。In order to optimize the disinfection system in tissue culture of wild Platycodon grandiflorum(Jacq.) A. DC. from Jilin Xianghai National Nature Reserve, NaClO disinfection method was used to determine the sterilization concentrations, sterilization time and culturing temperature of getting aseptic seedlings;the methods of hormone-seed soaking and heat-shock treatment were used to select the factors of hormone types, soaking concentrations as well as treating temperature and time, which affect the conditions for seed germination of wild Platycodon grandiflorum(Jacq.) A. DC.. Results showed that the optimum conditon to get aseptic seedling system of wild Platycodon grandiflorum(Jacq.) A. DC.was composed of soaking seeds by sterile wate, heat shocking treatment at 55 ℃ for 10 min, disinfected treatment in NaClO(1%) for 20 min and cultivated under visible light condition at 15 ℃, by which the regeneration rate of aseptic seedlings was 90.00%. The resnlts indicated that high levels seed germinating percentage and high quality aseptic seedlings of wild Platycodon grandiflorum(Jacq.) A. DC. could be got by means of dormancy-release method with disinfection treatments.

关 键 词：野生翠雀 种子 解休眠 消毒 无菌苗 

分 类 号：S339.41[农业科学—作物遗传育种] Q142.7[农业科学—农艺学]