基于导模共振效应的免标记DNA生物传感器  

Label free guided-mode resonance biosensor for DNA detection

作　　者：孙光瑀 曹曙桦 李瑞 王琦[1] SUN Guangyu;CAO Shuhua;LI Rui;WANG Qi(School of Optical-Electrical and Computer Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China)

机构地区：[1]上海理工大学光电信息与计算机工程学院,上海200093

出　　处：《光学仪器》2021年第1期28-33,共6页Optical Instruments

摘　　要：为了设计用于检测DNA杂交状态的生物传感器,基于严格耦合波理论和等效介质的数值计算方法,通过FDTD软件的模拟仿真优化,设计了在可见光波段偏振无关、高灵敏度,可以检测DNA杂交情况的透射型导模共振免标记生物传感器。当检测到单链DNA(ssDNA)时,共振波长为481.46 nm;当检测到双链DNA(dsDNA)时,共振波长为490.11 nm;当检测区域没有DNA存在时,无透射光出现,可以用于区分单双链DNA。该生物检测传感器能够保持样品的生物活性,避免样品的物理或化学损伤,便于多次分析检测,在生物检测领域有着潜在的应用价值。To design a bio-sensor for DNA detecting,we proposed a polarization free transmission type guided mode resonance biosensor with high sensitivity in visible wavelength was proposed for DNA detection with the FDTD software simulation based on the rigorous coupled wave theory and equivalent theory.When ssDNA was detected,the resonance wavelength was 481.46 nm.When dsDNA was detected,the resonance wavelength was 490.11 nm.When no DNA is present in the detection area,no transmitted light appears.Hence,it can be used to distinguish between single and double-stranded DNA.This bio-sensor can maintain the biological activity of the sample and avoid physical or chemical damage of the sample,which is convenient for multiple analysis and detection,and owns potential applications in the field of biological detection.

关 键 词：导模共振 生物传感器 免标记 偏振 耦合波理论 

分 类 号：O436[机械工程—光学工程]