纳米金-曙红Y体系荧光增强法检测异烟肼  

Determination of isoniazid by enhancement fluorescence method based on gold nanoparticles-eosin Y system

作　　者：申宇茫 田锐 高闯 马红燕 张攀 SHEN Yumang;TIAN Rui;GAO Chuang;MA Hongyan;ZHANG Pan(School of Chemistry and Chemical Engineering,Yan’an University,Yan’an 716000;Yan’an Key Laboratory of Analytical Technology and Detection,Yan'an 716000)

机构地区：[1]延安大学化学与化工学院,延安市分析技术与检测重点实验室,延安716000

出　　处：《分析试验室》2021年第2期203-206,共4页Chinese Journal of Analysis Laboratory

基　　金：延安大学博士科研启动项目(YDBK2017-05)资助。

摘　　要：基于异烟肼对纳米金-曙红Y体系的荧光增强作用,通过测定加入异烟肼前后体系荧光信号变化与异烟肼含量的关系,建立测定异烟肼的荧光分析方法。并探讨了实验机理,在优化的实验条件下,方法的线性范围为0.02~2.0μg/mL,检出限为9 ng/mL。对异烟肼片中异烟肼含量进行了测定,测定值与标识值没有显著差异,加标回收率在96.0%~103.0%之间。Based on the fluorescence enhancement effect of isoniazid to the gold nanoparticles-eosin Y system,a fluorescence analysis method for the determination of isoniazid was presented.The mechanism of the detection system,and the effect of experimental factors were studied.In the optimized conditions,the linear range of the method was 0.02-2.0μg/mL,with the detection limit of 9 ng/mL.The content of isoniazid in isoniazid tablets was quantitative measured.The results showed that there was no significant difference between measured and identified values,and the recoveries were between 96.0%and 103.0%.

关 键 词：纳米金 曙红Y 荧光增强 异烟肼 

分 类 号：O652[理学—分析化学] O657.3[理学—化学]