基于群体效应研究双氢杨梅树皮素对金黄色葡萄球菌的体内抗菌作用  

In-vivo Antibacterial Effects of Ampelopsis on Staphylococcus Aureus Based on Quorum Sensing

作　　者：洪正善 王元霞 杨柯[1] 曾春晖[1] HONG Zhengshan;WANG Yuanxia;YANG Ke;ZENG Chunhui(Guangxi University of Chinese Medicine,Nanning 530200,China;Guangxi International Zhuang Medicine Hospital)

机构地区：[1]广西中医药大学,广西南宁530200 [2]广西国际壮医医院

出　　处：《西部中医药》2021年第1期15-19,共5页Western Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(81460626,81060357);广西研究生教育创新计划学位与研究生教育改革课题研究项目(JGY2014086);广西研究生教育创新计划资助项目(YJSZ201704);广西中医药大学硕士研究生研究生创新项目(YCSY2018013)。

摘　　要：目的:探讨双氢杨梅树皮素(ampelopsis,APS)通过群体效应(quorum sensing,QS)对体内金黄色葡萄球菌(staphylococcus aureus,SA)的抗菌作用。方法:对标准SA NTCC8325及其QS调控基因缺失株NTCC8325ΔagrA、NTCC8325luxS进行生长曲线测定,以确定3个株菌的活力最强时期;检测高、中、低剂量APS给药后3个株菌腹腔感染小鼠腹腔液活菌数和小鼠血液白细胞数。结果:标准SANTCC8325活力最强时期为10~12 h,NTCC8325ΔagrA、NTCC8325ΔluxS活力最强时期为12~14 h;APS对3个株菌腹腔感染小鼠均有保护作用,且在一定范围内呈量效关系;APS对NTCC8325ΔluxS腹腔感染小鼠的保护作用较APS对NTCC8325ΔagrA腹腔感染小鼠的保护作用强。结论:APS在体内通过直接影响agr QS系统及间接影响lux QS系统,从而产生抗SA的作用。Objective:To explore antibacterial effects of APS on SA in vivo based on QS.Methods:The growth curves of standard SA NTCC8325 and its QS regulatory gene delection strains NTCC8325ΔagrA and NTCC8325luxS were determined,to confirm the most vigorous period of three strains;to measure the number of viable bacteria in peritoneal fluid of mice with abdominal infection and the number of white blood cells after administering with high,moderate and low dosages of APS.Results:The most vigorous period of standard SA NTCC8325 was from 10 to 12 hours,the periods of NTCC8325ΔagrA and NTCC8325luxS from 12 to 14 hours;APS owns protective effects on the mice suffering from three strains of bacteria infection in abdominal cavity,and it presented the dose-dependent relationship within a certain range;protective effects of APS on the mice with abdominal infection of NTCC8325ΔluxS were stronger compared with its effects on the mice with abdominal infection of NTCC8325ΔagrA.Conclusion:APS could produce anti-SA effects through directly affecting agr QS system and lux QS system indirectly in vivo.

关 键 词：金黄色葡萄球菌 致病性 双氢杨梅树皮素 体内抗菌 群体效应 

分 类 号：R978[医药卫生—药品]