创伤生肌水凝胶对动物皮肤创伤的愈合作用  

Study on the effect of traumatic myogenic hydrogel in promoting the healing of skin wound in rats

作　　者：梁卫 董国刚 刘梅[3] 顾萌 季敏 吴艮艮 梁涛[4] LIANG Wei;DONG Guo-gang;LIU Mei;GU Meng;JI Min;WU Gen-gen;LIANG Tao(Department of Traditional Chinese Medicine,Air Force Hospital,Eastern Theater of the Chinese People′s Liberation Army,Nanjing 210002,Jiangsu,China;Department of Radiology of the Second Outpatient,General Hospital of Eastern Theater Command,PLA,Nanjing 210002,Jiangsu,China;Department of Pharmacy,Air Force Hospital,Eastern Theater of the Chinese People′s Liberation Army,Nanjing 210002,Jiangsu,China;School of Pharmacy,Nanjing University of Traditional Chinese Medicine,Nanjing 210023,Jiangsu,China)

机构地区：[1]东部战区空军医院中医科,南京210002 [2]东部战区空军医院药剂科,南京210002 [3]东部战区总医院(原南京军区南京总医院)第二门诊部放射科,南京210002 [4]南京中医药大学药学院,南京210023

出　　处：《东南国防医药》2021年第2期117-121,共5页Military Medical Journal of Southeast China

基　　金：南京军区医学科技创新重点课题(14ZD26)。

摘　　要：目的研究外用创伤生肌水凝胶对小鼠皮肤创伤性溃疡和大鼠皮肤机械性创伤模型的影响。方法采用皮下注射稀盐酸造成小鼠创伤性溃疡模型和手术剪造成大鼠背部脊柱两侧圆形创面的大鼠机械性创伤模型,按随机区组原则分为5组:模型组、基质组、凝胶低剂量组、凝胶高剂量组、阳性组,每组各10只。模型组涂抹等渗盐水,基质组涂抹0.5%卡波姆凝胶基质,凝胶低剂量组涂抹1 g/mL创伤生肌水凝胶,凝胶高剂量组涂抹3.5 g/mL创伤生肌水凝胶,阳性组涂抹锦虹创面修复诱导凝胶,利用透明薄膜描记创面面积,计算小鼠和大鼠的创面愈合率;HE染色观察各组大鼠皮肤病理变化;ELISA法检测各组大鼠转化生长因子β1(TGF-β1)、白细胞介素-2(IL-2)、肿瘤坏死因子-α(TNF-α)含量;Western blot法检测各组大鼠核因子κB(NF-κB)、Smad2蛋白、Smad3蛋白的表达。结果凝胶高、低剂量组对小鼠创伤性溃疡模型的溃疡面积没有明显作用,但创伤生肌水凝胶可提高对大鼠机械性创伤的创面愈合率,给药第6天,凝胶低剂量组和高剂量组的创面愈合率分别为(42.6±0.30)%、(45.8±0.08)%,较基质组[(24.0±0.10)%]均明显提高(P<0.01);给药第9天,凝胶低剂量组和高剂量组的创面愈合率分别为(64.7±0.62)%、(75.5±0.71)%,较基质组[(53.6±0.15)]%均明显提高(P<0.01)。凝胶高、低剂量组皮肤组织病理学变化明显改善。凝胶高剂量组TGF-β1含量[(57.61±4.05)pg/mL]较基质组[(47.14±3.55)pg/mL]显著升高(P<0.05),组织蛋白NF-κB表达显著降低。结论创伤生肌水凝胶外用对大鼠机械性皮肤创伤愈合有较好的治疗作用,可能与细胞因子TGF-β1和NF-κB的蛋白水平改变有关。Objective To investigate the effect oftraumatogenic hydrogel on traumatic skin ulcers in mice and mechanical skin trauma models in rats.Methods A mouse model of traumatic ulcer was caused by subcutaneous injection of dilute hydrochloric acid and a rat mechanical trauma model was caused by surgical scissors with circular wounds on both sides of the back spine of rats.The models were divided into five groups according to the principle of randomized block,including model group,matrix group,trau-matogenic hydrogel low-dose group and traumatogenic hydrogel high-dose group.The model group was coated with isotonic saline.The matrix group was coated with 0.5%carbomer gel matrix.The trauma myogenic hydrogel low-dose group was coated with trauma 1 g/mL myogenic hydrogel.The trauma myogenic hydrogel high-dose group was coated with 3.5 g/mL myogenic hydrogel.The drug positive group was coated with Jinhong trauma repair inducing gel.The trauma area was marked and traced using transparent film calculating the trauma healing rates of mice and rats.HE staining was used to observe the effect of skin pathological changes in each group.ELISA was used to detect the content of transforming growth factorβ1(TGF-β1),interleukin-2(IL-2)and tumor necrosis factor-α(TNF-α)in each group.Western blot was used to detect the expression of nuclear factorκB(NF-κB),Smad2 protein and Smad3 protein in each group.Results All doses of traumatogenic hydrogel had no significant effects on the ulcer area of mice in traumatic ulcer models,however,it could improve the wound healing rates of mechanical trauma in rats.For 6 days′administration,the healing rate of traumatogenic hydrogel low dose group and high dose group were(42.6±0.30)%,(45.8±0.08)%,respectively,were significantly improved(P<0.01)compared with the matrix group(24.0±0.10)%.For 9 days′administration,the healing rates of the low-dose and high-dose groups of traumatogenic hydrogel were(64.7±0.62)%,(75.5±0.71)%,significantly improved(P<0.01)compared with the matrix group(53.6±0.15

关 键 词：皮肤 创伤生肌水凝胶 机械性创伤 核因子κB 转化生长因子β1 

分 类 号：R285.5[医药卫生—中药学]