四川省攀西地区羊梨形虫的分子流行病学调查  

Molecular epidemiological survey of ovine and caprine infection with piroplasms in Panxi region of Sichuan province

作　　者：郝桂英[1] 杨丽静 李瑞琪 黄秋连 郭嘉玲 杨应东[2] 万洁[2] 文建国[2] 徐睿[1] HAO Gui-ying;YANG Li-jing;LI Rui-qi;HUANG Qiu-lian;GUO Jia-ling;YANG Ying-dong;WAN Jie;WEN Jian-guo;XU Rui(School of Animal Science of Xichang College,Xichang 615013,China;Animal Science and Fisheries Research Institute,Panzhihua Academy of Agricultural and Forestry Sciences,Panzhihua 617061,China)

机构地区：[1]西昌学院动物科学学院,四川西昌615013 [2]四川省攀枝花市农林科学研究院畜牧水产所,四川攀枝花617061

出　　处：《中国预防兽医学报》2021年第1期92-97,共6页Chinese Journal of Preventive Veterinary Medicine

基　　金：四川省科技厅项目(2018JY0229);四川省教育厅项目(16ZA0267);西昌学院两高项目(LGLZ201808);攀西动物疫病检测与防控四川省高校重点实验室基本科研业务费项目(341B);四川地方特色山羊工程中心四川省高校重点实验室基本科研业务费项目。

摘　　要：为了解四川省攀西地区羊梨形虫的感染情况及种类分布,本研究采用原虫、泰勒虫18S rRNA基因通用引物和种特异性引物对采集自凉山州和攀枝花市7个县/市的497份羊血样样品进行套式PCR检测,并对阳性样品进行测序和序列分析。结果显示,攀西地区7个县/市羊梨形虫阳性率为11.27%(56/497),其中吕氏泰勒虫、尤氏泰勒虫阳性率分别为10.46%(52/497)、0.20%(1/497),混合感染率为0.20%(1/497),未检出绵羊泰勒虫。分歧巴贝斯虫和Babesia sp.KO1阳性率分别为0.20%(1/497)和0.80%(4/497),Babesia sp.KO1与吕氏泰勒虫混合感染率为0.20%(1/497),未检出莫氏巴贝斯虫和羊巴贝斯虫。山羊和绵羊梨形虫阳性率分别为10.35%(47/454)和20.93%(9/43)。经卡方检验,西昌市、布拖县、会理县和米易县梨形虫阳性率均与美姑县、普格县和盐边县差异显著(p<0.05),山羊与绵羊梨形虫阳性率也差异显著(p<0.05)。本研究结果表明攀西地区存在羊梨形虫的感染,且以单一病原感染为主,其中吕氏泰勒虫为主要虫种,该地区应加强对羊梨形虫病的防控。In order to understand the infection status and species distribution of ovine and caprine piroplasms in Panxi,Sichuan province,497 blood samples of ovine and caprine from seven counties in Liangshan Prefecture and Panzhihua City were detected using a nested polymerase chain reaction(PCR)based on universal primers and species-specific primers of 18S rRNA gene,and the positive samples of Theileria spp.and Babesia spp.were further sequenced and analyzed.The results showed that the positive rate of piroplasms was 11.27%(56/497),the positive rate of T.luwenshuin and T.uilenbergi was 10.46%(52/497)and 0.20%(1/497),respectively.Co-infection rate of T.luwenshuin and T.uilenbergi was 0.20%(1/497).T.ovis was not detected.The positive rate of B.divergens and Babesia sp.KO1 was 0.20%(1/497)and 0.80%(4/497),respectively.Co-infection rate of Babesia sp.KO1 and T.luwenshuin was 0.20%(1/497).B.motasi and B.ovis were not detected.The positive rate of piroplasms in caprine and ovine was 10.35%(47/454)and 20.93%(9/43),respectively.By X2 test,the positive rate of piroplasms in Xichang,Butuo,Huili,and Miyi was statistically significant different from Meigu,Puge and Yanbian county(p<0.05),respectively.And the positive rate of piroplasms in caprine was statistically significant different from the positive rate of piroplasms in ovine(p<0.05).These findings indicated that ovine and caprine infection with piroplasms existed in Panxi region,was mainly caused by a single pathogen,and T.luwenshuin was the main species.Therefore,prevention and control of ovine and caprine piroplasmos is should strengthen in this region.

关 键 词：梨形虫 羊 攀西地区 分子流行病学 18S rRNA 

分 类 号：S852.7[农业科学—基础兽医学]