基于双硫键的谷胱苷肽荧光传感器分子制备  

The Preparation of Fluorescent Sensor for Detecting Glutathione based on Disulfide Bond

作　　者：张晓琳 乔威威 马思阳 ZHANG Xiao-lin;QIAO Wei-wei;MA Si-yang(College of Environmental and Chemical Engineering,Dalian University,Dalian 116622,China)

机构地区：[1]大连大学环境与化学工程学院,辽宁大连116622

出　　处：《大连大学学报》2020年第6期89-92,共4页Journal of Dalian University

基　　金：国家自然科学基金(21102014);辽宁省自然科学基金指导计划项目(201602036)。

摘　　要：设计并合成了基于荧光共振能量转移机理的谷胱苷肽荧光传感器D-S-CR,该分子以双硫键作为谷胱苷肽识别位点,以蓝色荧光团香豆素衍生物作为荧光供体,黄色荧光团丹磺酰胺作为荧光受体,通过调节两个不同能量荧光团在识别谷胱苷肽前后距离的变化,实现了荧光传感器D-S-CR识别谷胱苷肽后荧光从黄到蓝的可视化检测。荧光传感器D-S-CR在谷胱苷肽比率荧光检测中具有良好的应用前景,有望对生物体内谷胱苷肽的识别提供更多有价值的信息。A glutathione fluorescent sensor D-S-CR based on fluorescence resonance energy transfer mechanism was designed and synthesized,which includes the disulfide bond as the glutathione recognition site,a blue-emitting coumarin derivative as the fluorescence donor,and a yellow-emitting dansyl amide as the fluorescence receptor.By adjusting the distance between two fluorophores before and after detecting glutathione,the fluorescence of D-S-CR changed obviously from yellow to blue.Fluorescent sensor D-S-CR would provide more valuable information for the recognition of glutathione in organisms in the future.

关 键 词：谷胱苷肽 双硫键 荧光传感器 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]