干露胁迫对脊尾白虾存活率及氧化应激反应的影响  

Effects of Air Exposure Stress on Survival Rate and Response to OxidativeStress in Ridgetail White Prawn Exopalaemon carinicauda

作　　者：邓高威 段健诚 王玉 王林华 欧阳乐飞 高焕 赖晓芳[1,2,3] 张庆起 阎斌伦[1,2,3] DENG Gaowei;DUAN Jiancheng;WANG Yu;WANG Linhua;OUYANG Lefei;GAO Huan;LAI Xiaofang;ZHANG Qingqi;YAN Binlun(College of Marine Sciences and Fisheries,Jiangsu Key Laboratory of Marine Biotechnology,Jiangsu Ocean University,Lianyungang 222005,China;Jiangsu Institute of Marine Resources Development,Lianyungang 222005,China;The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm,Nanjing 210014,China;Ganyu-Jiaxin Aquaculture Limited Company of Lianyungang,Lianyungang 222100,China)

机构地区：[1]江苏海洋大学海洋科学与水产学院,江苏省海洋生物技术重点实验室,江苏连云港222005 [2]江苏省海洋资源开发研究院,江苏连云港222005 [3]江苏省农业种质资源保护与利用平台,江苏南京210014 [4]连云港赣榆佳信水产开发有限公司,江苏连云港222100

出　　处：《水产科学》2021年第2期244-249,共6页Fisheries Science

基　　金：江苏省高等学校自然科学研究重大项目(17KJA240001);江苏省研究生科研与实践创新计划项目(SJCX19-0987).

摘　　要：为探究干露胁迫对脊尾白虾存活率和氧化应激反应的影响,设置常温干燥、常温湿润、低温干燥和低温湿润4个胁迫试验组及1个对照组,计算各组胁迫后12.0 h内脊尾白虾累计存活率,并分析各组胁迫后2.5 h内肌肉中过氧化氢酶、谷胱甘肽过氧化物酶、超氧化物歧化酶活性和丙二醛含量变化。试验结果显示,各组累计存活率差异显著( P <0.05),3.0 h常温干燥组个体全部死亡,常温湿润、低温干燥和低温湿润等3个试验组的累计存活率分别为6.7%、38.9%和40.0%。4个试验组过氧化氢酶活性和谷胱甘肽过氧化物酶活性均显著高于对照组( P <0.05);超氧化物歧化酶活性呈先降后升趋势,在1.0 h和2.5 h时显著升高( P <0.05);丙二醛含量呈先升后降趋势,在1.0 h和 1.5 h时显著升高( P <0.05)。试验结果表明,低温湿润可以提高脊尾白虾存活率,干露环境条件可诱导脊尾白虾产生氧化应激反应,过氧化氢酶、谷胱甘肽过氧化物酶、超氧化物歧化酶活性和丙二醛含量变化明显,可作为干露条件下脊尾白虾机体健康监测指标。The objective of present research was to investigate the effects of air exposure stress on survival rate and response to oxidative stress in ridgetail white prawn Exopalaemon carinicauda . The shrimps were grouped to control treatment and four experimental treatments exposed to four challenges: room temperature and air exposure, room temperature and wet, low temperature and air exposure, low temperature and wet. The accumulative survival rates were calculated within 12.0 h, and the antioxidant enzyme activities were measured within 2.5 h under air exposure stress. The results showed that the accumulative survival rates of ridgetail white prawn in the groups were significantly different. At 3.0 h, all individuals in room temperature and air exposure were dead, while the other three test groups (room temperature and wet, low temperature and air exposure, and low temperature and wet) were 6.7% , 38.9% and 40.0% respectively. Moreover, the activities of catalase (CAT) and glutathione peroxidase (GPx) in the four test groups were significantly higher than those in the control group( P <0.05). In addition, the activities of superoxide dismutase (SOD) were decreased initially and then significantly increased at 1.0 and 2.5 h ( P < 0.05 ). But, the contents of malonaldehyde (MDA) were significantly increased initially at 1.0 and 2.5 h ( P <0.05) and then decreased. These findings indicated that low and wet temperature improved the survival rates of ridgetail white prawn, the response to oxidative stress of ridgetail white prawn were induced by air exposure stress. Activities of CAT, GPx, SOD and MDA were changed significantly, which can be used as indicators for monitoring the health of ridgetail white prawn exposed to air exposure conditions.

关 键 词：存活率 抗氧化酶 丙二醛 脊尾白虾 干露胁迫 

分 类 号：S968.22[农业科学—水产养殖]