结核感染T细胞斑点试验在手、腕部腱鞘结核诊断中的应用评价  

Evaluation of the T-SPOT.TB test for diagnosis of Tuberculous tenosynovitis of the hand and wrist

作　　者：曾锦源[1] 林章雄[1] 谢昀[1] 陈春永[1] 叶君健[1] ZENG Jin-yuan;LIN Zhang-xiong;XIE Yun;CHEN Chun-yong;YE Jun-jian(Department of Orthopedic Trauma,Fujian Trauma Medical Center,First Affiliated Hospital of Fujian Medical University,Fuzhou 350005,China)

机构地区：[1]福建医科大学附属第一医院创伤骨科/福建省创伤医学中心,福州350005

出　　处：《中国人兽共患病学报》2021年第2期193-196,共4页Chinese Journal of Zoonoses

基　　金：福建教育厅立项科研项目(No.JAT190209)。

摘　　要：目的探讨结核感染T细胞斑点试验(T-cell spot of Tuberculosis,T-SPOT.TB)在手、腕部腱鞘结核诊断中的临床应用价值。方法通过对2012年1月至2018年1月我院收集64例怀疑手、腕部腱鞘结核临床资料进行回顾性分析,根据病理诊断及临床表现判断有无结核分枝杆菌感染;全部病例均经过T-SPOT.TB和胶体金标法分别进行体外γ-干扰素释放试验和血清结核抗体检测,采用Kappa、χ^(2)检验进行统计学分析。结果共收集分析相关病例64例,其中诊断为手、腕部腱鞘结核(结核病例)21例,非手、腕部腱鞘结核(非结核病例)43例。经T-SPOT.TB检测,结核病例阳性者20例,非结核病例阴性者40例,灵敏度为95.2%,特异度为93.0%,阳性预测值为87.6%,阴性预测值为97.6%。经胶体金标法检测血清结核抗体,结核病例阳性者5例,非结核病例阴性者37例,灵敏度为23.8%,特异度为86.0%,阳性预测值为45.4%,阴性预测值为69.8%。与临床诊断比较,T-SPOT.TB和胶体金标法的Kappa值分别为0.862和0.112,T-SPOT.TB比胶体金标法具有更好的一致性,且灵敏度、阳性预测值和阴性预测值均明显更高,差异有统计学意义(P<0.05)。结论T-SPOT.TB在手、腕部腱鞘结核诊断中具有良好的辅助诊断价值。To evaluate the clinical application of the T-SPOT.TB test in the diagnosis of Tuberculous tenosynovitis of the hand and wrist,we retrospectively analyzed the clinical information for 64 suspected cases of Tuberculous tenosynovitis of the hand and wrist treated in our hospital between January 2012 and January 2018.Pathological diagnosis and clinical manifestations were used for the diagnosis of tuberculosis infection.All patient samples were assayed with T-SPOT.TB,the colloidal gold method,interferon gamma release assays and serum tuberculosis antibody detection.All data statistical analyses were performed with Kappa and Chi-square tests.A series of 64 related cases were analyzed,among which 21 patients were diagnosed with Tuberculous tenosynovitis of the hand and wrist(tuberculous cases),and 43 patients were diagnosed with non-tuberculosis tenosynovitis of the hand and wrist(non-tuberculous cases).The T-SPOT.TB test revealed 20 cases positive for tuberculosis and 40 cases negative for tuberculosis,with 95.2%sensitivity and 93.0%specificity.The positive and negative predictive value of the test was 87.6%and 97.6%,respectively.In serum tuberculosis antibody tests with the colloidal gold method test,5 positive tuberculosis cases and 37 negative tuberculosis cases were found,with 23.8%sensitivity and 86.0%specificity;the positive and negative predictive value was 45.4%and 69.8%,respectively.Additionally,the Kappa values for the T-SPOT.TB test and colloidal gold method were 0.862 and 0.112,respectively,thus indicating that T-SPOT.TB showed better consistency,and higher sensitivity and positive and negative predictive value,than the colloidal gold method(P<0.05).T-SPOT.TB thus has good supplementary diagnostic value for tuberculous tenosynovitis of the hand and wrist.

关 键 词：手和腕部腱鞘结核 结核感染T细胞斑点试验 Γ-干扰素释放试验 胶体金标法 结核抗体 

分 类 号：R378.91[医药卫生—病原生物学] R658.2[医药卫生—基础医学]