α-平滑肌肌动蛋白、上皮钙黏素在腹膜透析大鼠腹膜组织中的表达及意义  

Expression and significance ofα-SMA and E-cadherin in peritoneal tissues of rats undergoing peritoneal dialysis

作　　者：彭霞[1] 徐家云[1] 王朋朋[2] PENG Xia;XU Jiayun;WANG Pengpeng(Kidney Internal Medicine,The First Affiliated Hospital of Henan University of Science and Technology,Luoyang,Henan 471000,China;Cardiovascular Medicine,The First Affiliated Hospital of Henan University of Science and Technology,Luoyang,Henan 471000,China)

机构地区：[1]河南科技大学第一附属医院肾脏内科,河南洛阳471000 [2]河南科技大学第一附属医院心血管内科,河南洛阳471000

出　　处：《安徽医药》2021年第3期586-590,I0006,共6页Anhui Medical and Pharmaceutical Journal

基　　金：2016河南省科技攻关计划项目(162102310216)。

摘　　要：目的探讨α-平滑肌肌动蛋白(α-SMA)、上皮钙黏素(E-cadherin)在腹膜透析大鼠腹膜组织中的表达及意义。方法SPF级SD大鼠45只,抽签选取26只采用5/6肾切除法制备大鼠尿毒症模型,将造模成功的21只再抽签选取20只分为模型组和腹膜透析组各10只;另剩余19只大鼠抽签选取10只作为假手术组。术后5周建立腹膜透析模型;腹膜透析组每天灌注4.25%腹膜透析液20 mL,持续6周。停止透析24 h后测定腹膜超滤量、透出液肌酐(D-Scr)、血肌酐(P-Scr)、初始腹透液糖(D0)及透出液糖(D1)浓度;苏木精-伊红(HE)和Masson染色观察腹膜组织病理变化;反转录聚合酶链反应(RT-PCR)和免疫组化测定腹膜组织α-SMA、E-cadherin蛋白和mRNA的表达;Pearson相关性分析α-SMA和E-cadherin表达量之间的相关性,α-SMA、E-cadherin与腹膜结构和功能之间的相关性。结果与模型组相比,腹膜透析组大鼠的腹膜超滤量和D1/D0明显下降(P<0.05),D/P-Scr、腹膜厚度明显增加(P<0.05);与假手术组相比,模型组和腹膜透析组大鼠α-SMA的mRNA水平明显上调[(0.56±0.05),(0.92±0.09),(1.38±0.12),P<0.05],E-cadherin的mRNA水平明显下调[(0.41±0.04),(0.35±0.04),(0.24±0.03),P<0.05],且相比模型组,腹膜透析组大鼠α-SMA的mRNA水平明显上调,E-cadherin的mRNA水平明显下调(P<0.05),各组大鼠α-SMA和E-cadherin的mRNA水平呈负相关关系(P<0.05);与假手术组相比,模型组和腹膜透析组大鼠α-SMA的蛋白表达明显上调[(0.42±0.05),(0.83±0.08),(1.25±0.11),P<0.05],E-cadherin的蛋白表达明显下调[(0.32±0.04),(0.28±0.03),(0.21±0.03),P<0.05],且相比模型组,腹膜透析组大鼠α-SMA的蛋白表达明显上调,E-cadherin的蛋白表达明显下调(P<0.05),各组大鼠α-SMA和E-cadherin的蛋白表达呈负相关关系(P<0.05);大鼠腹膜组织中α-SMA的mRNA水平与腹膜厚度和D/P-Scr呈正相关关系(P<0.05),与腹膜超滤量和D1/D0呈负相关关系(P<0.05),E-cadherin与腹膜厚度和D/P-Scr呈Objective To explore expression and significance ofα-smooth muscle actin(α-SMA)and E-cadherin in peritoneal tissue of rats undergoing peritoneal dialysis(PD).Methods Of 45 SPF SD rats,26 rats were selected by lottery,and 21 of them were success⁃fully obtained by modeling.Twenty of them were divided into model group and peritoneal dialysis group with 10 each.The remaining 19 rats were selected by lottery as sham operation group.In model group and PD group,rat uremia models were established by 5/6 ne⁃phrectomy.At 5 weeks after surgery,PD models were established.PD group was perfused with 4.25%peritoneal dialysate of 20 mL per day for 6 weeks.At 24h after stopping dialysis,Peritoneal ultrafiltration volume,concentration of dialysate creatinine(D-Scr),plasma creatinine(P-Scr),initial peritoneal dialysate glucose(D0)and dialysate glucose(D1)were measured.HE and Masson staining were ap⁃plied to observe pathological changes of peritoneal tissues.The expressions ofα-SMA,E-cadherin protein and mRNA in peritoneal tis⁃sues was detected by RT-PCR and immunohistochemistry.The correlation betweenα-SMA and E-cadherin expression,correlation be⁃tweenα-SMA,E-cadherin and peritoneal structure and function were analyzed by Pearson correlation analysis.Results Compared with model group,peritoneal ultrafiltration volume and D1/D0 were significantly decreased in PD group(P<0.05),while D/P-Scr and peritoneal thickness were significantly increased(P<0.05).Compared with the sham operation group,the mRNA level ofα-SMA in the model group and peritoneal dialysis group was significantly increased[(0.56±0.05),(0.92±0.09),(1.38±0.12),P<0.05],and the mRNA level of E-cadherin was significantly decreased[(0.41±0.04),(0.35±0.04),(0.24±0.03),P<0.05],The mRNA levels ofα-SMA and E-cadherin were negatively correlated in each group(P<0.05);compared with the sham operation group,the protein expres⁃sion ofα-SMA in the model group and peritoneal dialysis group was significantly increased[(0.42±0.05),(0.83±0.08),(1.25±0.11),P<0.

关 键 词：尿毒症 腹膜透析 Α-平滑肌肌动蛋白 上皮钙黏素 大鼠 Sprague-Dawley 

分 类 号：R692.5[医药卫生—泌尿科学]