复方麝香膏治疗外阴硬化性苔癣的临床疗效及对细胞周期蛋白D1、抑癌基因p16表达的影响  

The clinical effect of compound musk ointment on vulva sclerosing lichen and its effect on the expression of cyclin D1,tumor suppressor gene p16 in the treatment of vulvar lichen sclerosus by compound musk ointment

作　　者：郭佩[1] 周英惠[2] 卿荣珍 GUO Pei;ZHOU Yinghui;QING Rongzhen(Department of Gynaecology,International Zhuang Medical Hospital Affiliated to Guangxi University of Traditional Chinese Medicine,Nanning,Guangxi 530000,China;Department of Gynaecology,Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine,Nanning,Guangxi 530000,China)

机构地区：[1]广西中医药大学附属国际壮医医院妇科,广西南宁530000 [2]广西中医药大学附属瑞康医院妇科,广西南宁530000

出　　处：《安徽医药》2021年第3期607-610,共4页Anhui Medical and Pharmaceutical Journal

摘　　要：目的探讨复方麝香膏治疗外阴硬化性苔癣的临床疗效及对病变组织中细胞周期蛋白D1(cyclin D1)及抑癌基因p16表达的影响。方法选取2017年6月至2019年6月广西中医药大学附属瑞康医院的90例病人,经病理活检确诊为外阴硬化性苔癣,按随机数字表法分为两组,观察组及对照组各45例,对照组选择常规传统药物治疗,观察组选用复方麝香膏外用,连续用药3个月。治疗前、治疗完成后即刻、治疗后6个月取外阴原病变处组织行病理学检查,并采用免疫组化方法检测病变组织中cyclin D1与p16的表达水平,于治疗完成后即刻、治疗后6个月判定两组的临床疗效并比较有效率。结果对照组与观察组治疗完成后即刻两组cyclin D1蛋白阳性表达率分别为31.11%、24.44%,与治疗前(71.11%、68.89%)比较均降低(P<0.05);p16蛋白阳性表达率分别为24.44%、26.67%,与治疗前(6.67%、8.89%)比较均升高(P<0.05)。治疗后6个月观察组cy⁃clin D1蛋白阳性表达率28.89%仍低于治疗前68.89%(P<0.05),p16蛋白阳性表达率24.44%高于治疗前8.89%(P<0.05),对照组cyclin D1及p16蛋白阳性表达率与治疗前差异无统计学意义(P>0.05)。治疗完成后即刻两组cyclin D1及p16蛋白阳性表达率均差异无统计学意义(P>0.05);治疗后6个月,观察组cyclin D1蛋白阳性表达率28.89%低于对照组51.11%(P<0.05),p16蛋白阳性表达率24.44%高于对照组8.89%(P<0.05)。治疗完成后即刻两组总有效率分别为75.6%、82.2%,差异无统计学意义(P>0.05),治疗后6个月观察组总有效率77.8%高于对照组57.8%(P<0.05)。结论复方麝香膏及糖皮质激素均是治疗外阴硬化性苔癣的有效方法,两者均能降低cyclin D1的表达水平,增加p16的表达水平,短期疗效相当,但复方麝香膏疗效稳定,治疗后6个月时复方麝香膏疗效优于糖皮质激素。Objective To investigate the clinical efficacy of compound musk ointment in the treatment of vulvar lichen sclerosus(VLS)and its effect on cyclin D1 and tumor suppressor gene p16 expression in diseased tissues.Methods A total of 90 cases with VLS diagnosed by pathological biopsy from June 2017 to June 2019 in Ruikang Hospital Affiliated to Guangxi University of Chinese Medicinel were randomly divided into the observation group and the control group,with 45 cases in each group.The control group was treated with conventional traditional drugs,and the observation group was treated with compound musk ointment for external use for 3 months.Histopathological examination was performed before,after and 6 months after treatment.The expressions of cyclin D1 and p16 in pathological tissues were detected by immunohistochemistry,and the clinical efficacy of the two groups was compared.Results The positive expression rates of cyclin D1 protein in the control group and the observation group immediately after treatment were 31.11%and 24.44%respectively,which were lower than those before treatment(71.11%and 68.89%)(P<0.05),and the positive ex⁃pression rates of p16 protein of the two groups after treatment were 24.44%and 26.67%,respectively,which were higher than those be⁃fore treatment(6.67%and 8.89%)(P<0.05).Six months after treatment,the positive expression rate of cyclinD1 protein was 28.89%,which was still lower than that before treatment(68.89%)(P<0.05);while the positive expression rate of p16 protein was 24.44%,which was higher than that before treatment(8.89%)(P<0.05).There was no difference between the positive expression rates of cyclin D1 and p16 protein in the control group at 6 months after treatment and those before treatment(P>0.05).There was no significant differ⁃ence in the positive expression rate of cyclin D1 and p16 protein between the two groups after treatment(P>0.05);after 6 months of treatment,the positive expression rate of cyclin D1 protein in the observation group(28.89%)was lower than that in the

关 键 词：外阴硬化性苔癣 复方麝香膏 氢化可的松 细胞周期蛋白D1 基因 p16 

分 类 号：R711.72[医药卫生—妇产科学]