Circ_0000003通过调控miR-338-3p/IRS2轴调控甲状腺癌细胞增殖、转移和凋亡  

Circ_0000003 regulates the proliferation, metastasis and apoptosis of thyroid carcinoma cells by regulating the miR-338-3p/IRS2 axis

作　　者：樊芳 关慧娟 FAN Fang;GUAN Hui-juan(Department of Breast and Thyroid Surgery,Yankuang New Mileage General Hospital,Jining 273500,Shandong Province,China;Department of Pathology,Yankuang New Mileage General Hospital,Jining 273500,Shandong Province,China)

机构地区：[1]兖矿新里程总医院乳甲外科,山东济宁273500 [2]兖矿新里程总医院病理科,山东济宁273500

出　　处：《中国临床药理学杂志》2021年第4期421-424,共4页The Chinese Journal of Clinical Pharmacology

摘　　要：目的探究circ0000003对甲状腺癌(TC)细胞增殖、转移和凋亡的影响及其机制。方法 CGTH W-3细胞分为转染组-1(转染空白质粒)、转染组-2(转染pcDNA-circ0000003)、转染组-3(共转染miR-338-3p mimics和pcDNA-circ0000003);TPC-1细胞分为转染组-4(转染control si-RNA)、转染组-5(转染si-circ0000003)、转染组-6(共转染miR-338-3p inhibitors和si-circ0000003)。用定量逆转录聚合酶链式反应(qRT-PCR)检测TC组织和细胞系中circ0000003表达。用蛋白质印迹法检测TC组织中胰岛素受体底物2(IRS2)及凋亡相关蛋白的表达,用CCK-8实验检测细胞增殖,用Transwell实验检测细胞迁移和侵袭能力,用双荧光素酶报告基因实验验证circ0000003与miR-338-3p之间的靶向关系。结果 Circ0000003在甲状腺癌组织(3.41±0.27)中的表达水平显著较癌旁组织(1.03±0.11)高(P<0.05)。转染组-1,转染组-2,转染组-4,转染组-5在96 h时吸光度分别为0.70±0.07,0.85±0.07,0.71±0.07和0.57±0.04;迁移细胞数目分别为(124.33±6.18),(171.00±12.19),(71.00±6.19)和(126.30±7.93)个;侵袭迁移细胞数目分别为(89.00±11.86),(126.33±7.93),(86.67±8.86)和(56.33±7.93)个。上述指标,转染组-2与转染组-1比较,转染组-5与转染组-4比较,差异均有统计学意义(均P<0.05)。结论 Circ0000003通过调节miR-338-3p/IRS2轴促进TC细胞的增殖、迁移和侵袭,并抑制细胞凋亡。Objective To explore the effect of circ0000003 on the proliferation, metastasis and apoptosis of thyroid carcinoma(TC) cells and its mechanism.Methods CGTH W-3 cells were assigned to Transfection-1 group(transfected with blank plasmid), Transfection-2 group(transfected with pcDNA-circ0000003) and Transfection-3 group(transfected with miR-338-3 p mimics and pcDNA-circ0000003).TPC-1 cells were assigned to Transfection-4 group(transfected with control Si RNA), Transfection-5 group-(transfected with si-circ0000003) and Transfection-6 group(miR-338-3 p inhibitors and si-circ0000003).Quantitative reverse transcription-polymerase chain reaction(qRT-PCR) was used to detect the expression of circ0000003 in TC tissues and cell lines.Western blot was used to detect the expression of insulin receptor substrate 2 (IRS2) and apoptosis-related proteins in TC tissues.CCK-8 assays were used to detect cell proliferation.Transwell assay was used to detect cell migration and invasion abilities.Dual-luciferase reporter gene assay was used to verify the targeted relationship between circ0000003 and miR-338-3 p.Results The expression of circ0000003 in TC tissues(3.41 ± 0.27) was significantly higher than that in adjacent tissues(1.03 ± 0.11),The difference has statistically significant(P < 0.05).The absorbances of Transfection-1 group,Transfection-2 group,Transfection-3 group and Transfection-5 group were 0.70 ± 0.07,0.85 ± 0.07,0.71 ± 0.07 and 0.57 ± 0.04,respectively;number of migrated cell were 124.33 ± 6.18,171.00 ± 12.19,71.00 ± 6.19 and 126.30 ± 7.93;number of invaded cell were 89.00 ± 11.86,126.33 ± 7.93,86.67 ± 8.86 and 56.33 ± 7.93.There were statistically significant differences between the Transfection-2 group and Transfection-1 group,Transfection-5 group and Transfection-3 group in the above indexes(P < 0.05).Conclusion Circ0000003 promotes the proliferation and metastasis of TC cells and inhibits cell apoptosis by regulating the miR-338-3 p/IRS2 axis.

关 键 词：circ_0000003 miR-338-3p 胰岛素受体底物2 甲状腺癌 

分 类 号：R97[医药卫生—药品]