毛蕊花苷通过miR-708-3p抑制乳腺癌MCF-7细胞的恶性表型  

Verbascoside inhibits the malignant phenotype of breast cancer MCF-7 cells by regulating miR-708-3p

作　　者：关慧娟 李涌 刘易强 樊芳 GUAN Hui-juan;LI Yong;LIU Yi-qiang;FAN Fang(Department of Pathology,Yankuang New Mileage General Hospital,Jining 273500,Shandong Province,China;Department of Pathology,Yanzhou District,Affiliated Hospital of Jining Medical College,Jining 272100,Shandong Province,China;Department of Pathology,Yanzhou District Railway Hospital,Jining 272100,Shandong Province,China;Department of Breast and Thyroid Surgery,Yankuang New Mileage General Hospital,Jining 273500,Shandong Province,China)

机构地区：[1]兖矿新里程总医院病理科,山东济宁273500 [2]济宁医学院附属医院兖州院区病理科,山东济宁272100 [3]济宁兖州区铁路医院病理科,山东济宁272100 [4]兖矿新里程总医院乳甲外科,山东济宁273500

出　　处：《中国临床药理学杂志》2021年第4期425-427,共3页The Chinese Journal of Clinical Pharmacology

摘　　要：目的探究毛蕊花苷对乳腺癌MCF-7细胞增殖、迁移、侵袭及上皮-间质转化(EMT)的影响。方法人乳腺癌MCF-7细胞随机分为对照组(生理盐水)以及实验组(毛蕊花苷50μmol·L-1)。给药24 h后,CCK-8法检测各组细胞存活率;Transwell实验检测各组细胞迁移和侵袭能力;Western blot检测EMT相关蛋白的表达。结果 72 h时,对照组和实验组的细胞存活率分别为(95.03±6.31)%和(80.11±5.17)%;细胞迁移数目分别为(91.03±9.37)和(58.69±5.08)个;细胞侵袭数分别为(83.47±7.88)和(42.86±4.91)个;E-钙粘蛋白相对表达量分别为0.73±0.07和1.27±0.11;B-钙粘蛋白相对表达量分别为0.88±0.08和0.53±0.04。实验组与对照组相比,差异均有统计学意义(P<0.01)。实验组miR-708-3p相对表达量显著高于对照组,差异有统计学意义(P<0.01)。结论毛蕊花苷通过上调miR-708-3p抑制人乳腺癌MCF-7细胞的增殖,迁移,侵袭和EMT。抑制肿瘤进展。Objective To investigate the effects of verbascoside(VER) on proliferation, migration, invasion and epithelial-mesenchymal transformation(EMT) of breast cancer MCF-7 cells.Methods Breast cancer MCF-7 cells were randomly divided into the control group(normal saline) and the test group(50 mol·L-1VER).After 24 h of administration, the cell survival rate of each group was determined by CCK-8 method.Transwell assay detected the ability of cell migration and invasion in each group.Western blot assay was used to detect the expression of EMT-related proteins.Results The cell survival rate of the control group and the test group was(95.03±6.31) % and(80.11±5.17) %, respectively.The number of cell migration was 91.03±9.37 and 58.69±5.08.The number of cell invasion was 83.47±7.88 and 42.86±4.91;The relative expression of E-cadherin were 0.73±0.07 and 1.27±0.11, respectively.The relative expression levels of B-cadherin were 0.88±0.08 and 0.53±0.04, respectively.The differences between the test group and the control group were statistically significant(P<0.05).The relative expression of miR-708-3 p in the test group was significantly higher than that in the control group.The difference was statistically significant(P<0.05).Conclusion VER inhibited the proliferation,migration,invasion and EMT of human breast cancer MCF-7 cells by upregulating miR-708-3 p.

关 键 词：毛蕊花苷 乳腺癌 miR-708-3p 迁移 侵袭 上皮-间质转化 

分 类 号：R28[医药卫生—中药学]