哮喘miRNA-mRNA调节网络的构建与小分子药物的预测  

Construction of miRNA-mRNA regulatory network for asthma and prediction of small molecule drugs

作　　者：邢西迁 杨姣[2] 白敏 杨娜[1] 和晓华[1] 黄孝娴 杨洁 刘洁 XING Xi-qian;YANG Jiao;BAI Min;YANG Na;HE Xiao-hua;HUANG Xiao-xian;YANG Jie;LIU Jie(Department of Respiratory Medicine,The Second People's Hospital of Yurnan Province,the Fourth Afiliated Hospital of Kunming Medical University,Kunming 650021,Yunnan Province,China;Department of Respiratory Medicine,The First Afiliated Hospital of Kunming Medical Universily,Kunming 650032,Yunnan Prorince,China)

机构地区：[1]云南省第二人民医院、昆明医科大学第四附属医院呼吸与危重症医学科,云南昆明650021 [2]昆明医科大学第一附属医院呼吸与危重症医学科,云南昆明650032

出　　处：《中国临床药理学杂志》2021年第4期454-457,共4页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金资助项目(81760015;81560694);云南省科技厅-昆明医科大学联合专项面上项目(2018FE001(-206));云南省高层次人才培养支持计划“名医”专项;云南省卫生高层次人才培养计划(H-2018095);云南省中青年学术和技术带头人后备人才(2017HB053);昆明医科大学研究生创新基金(2020S232)。

摘　　要：目的构建miRNA-mRNA网络,预测哮喘的治疗靶点和潜在的治疗药物。方法从基因表达综合数据库(GEO)下载2个数据集,用edgeR进行基因表达差异分析。差异miRNAs的靶点由miRTarBase、miRDB和TargetScan预测,通过cytoscape构建miRNA-mRNA网络。基于Metascape进行功能富集,cMAP用于预测小分子药物。结果筛选出118个差异mRNAs和16个差异miRNAs。在miRNA-mRNA网络中,miR-101-3p、miR-96-5p和miR-429可能成为哮喘的治疗靶点。功能富集发现差异mRNAs主要与免疫应答、炎症反应、MAPK级联调节和TGF-beta信号通路等有关。此外,我们发现了5种哮喘的潜在治疗药物。结论成功构建miRNA-mRNA网络,miR-101-3p、miR-96-5p和miR-429均可能为治疗靶点,并预测了5种哮喘的潜在治疗药物。Objective To construct the miRNA-mRNA regulatory network, so as to identify more reliable therapeutic targets and potential small molecule drugs.Methods Two datasets were downloaded from the gene expression omnibus(GEO), and the differently expressed(DE) analysis were conducted by edgeR.Functional enrichment analysis was performed using Metascape database.The targets of DE miRNAs were predicted by miRTarBase, miRDB and TargetScan databases, and the miRNA-mRNA regulatory network was constructed by Cytoscape software.Small molecule drugs were predicted on cMAP network.Results A total of 118 DE mRNAs and 16 DE miRNAs were identified.Then the miRNA-mRNA regulatory network was constructed.Among DE miRNAs in the network, miR-101-3 p, miR-96-5 p and miR-429 may be the most significant due to their large number of connecting nodes in asthma.The integrated DE genes were mainly concentrated in immune response, inflammatory response, regulation of MAPK cascade and TGF-beta signaling pathway.In addition, we found five potential treatments for asthma.Conclusion The miRNA-mRNA regulatory network was constructed,and miR-101-3 p, miR-96-5 p and miR-429 may be the possible therapeutic targets for asthma.In addition, our study provides four potential drugs for asthma.

关 键 词：哮喘 miRNA-mRNA网络 治疗靶点 小分子药物 

分 类 号：R97[医药卫生—药品]