低强度聚焦超声对缺血性脑卒中的干预作用  

Effect of low intensity ultrasound on ischemic stroke

作　　者：易沙沙 邹俊杰 冯相君 刘秀芳 马寅仲 畅君雷 孟龙[1] 敖丽娟 牛丽丽[1] Yi Shasha;Zou Junjie;Feng Xiangjun;Liu Xiufang;Ma Yinzhong;Chang Junlei;Meng Long;Ao Lijuan;Niu Lili(Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518055,China;The Rehabilitation School of Kunming Medical University,Kunming 650000,China)

机构地区：[1]中国科学院深圳先进技术研究院,518055 [2]昆明医科大学康复学院,650000

出　　处：《中华超声影像学杂志》2020年第12期1089-1094,共6页Chinese Journal of Ultrasonography

基　　金：国家自然科学基金(81527901,81660381,81960421);广东省重点领域研发计划项目(2018B030332001)。

摘　　要：目的通过搭建超声神经调控系统,并对超声换能器的声场进行表征,观察超声神经调控技术对大脑中动脉闭塞(MCAO)小鼠模型的干预作用。方法39只MCAO模型小鼠分为三组:MCAO组(13只)、假刺激组(13只)和超声刺激组(13只)。超声刺激组将中心频率为500 kHz的超声换能器作用于MCAO模型小鼠的脑梗死半球(本研究中为左侧),每次刺激时间为15 min,连续刺激5 d。在假刺激组中,将同样的超声换能器作用于脑梗死半球,但不打开功率放大器。在超声刺激后的第1 d、第3 d和第5 d进行TTC染色和旷场实验以评估小鼠的脑缺血体积和运动能力。结果与MCAO组和假刺激组相比,在超声刺激后的第3 d,超声刺激组模型小鼠的脑梗死体积占比百分数明显减少[MCAO组(23.45±1.99)%,假刺激组(22.43±3.07)%,超声刺激组(8.53±3.43)%,P<0.01],同时在超声刺激后第5 d观察到小鼠的运动总距离增加,但差异无统计学意义[MCAO组(1113.04±206.30)cm,假刺激组(944.08±206.60)cm,超声刺激组(1629.45±54.10)cm,P=0.07]。结论低强度聚焦超声作为一种神经调控技术可以显著减少缺血性脑卒中的脑损伤,同时对损伤后运动功能的恢复有一定的促进作用,因此在缺血性脑卒中的干预上有很大潜力。Objective To study the effect of ultrasound neuromodulation technology on middle cerebral artery occlusion(MCAO)mouse model through the establishment of an ultrasound neuromodulation system and the characterization of the acoustic field of ultrasound transducer.Methods Tirty-nine MCAO model mice were divided into three groups:MCAO group(13 mice),sham stimulation group(13 mice)and ultrasound stimulation group(13 mice).The ultrasound with a center frequency of 500 kHz was applied to the cerebral infarction hemisphere of MCAO model mice(left side in this study)in ultrasound stimulation group.The duration of ultrasound stimulation was 15min,and stimulation was done for 5 days.In the sham stimulation group,the same ultrasonic transducer was applied to the infarcted hemisphere without turning on the power amplifier.TTC staining and open field were performed on the first,third,and fifth days after ultrasound stimulation to assess cerebral ischemia volume and mobility of the mice.Results The percentage of cerebral infarction volume of the ultrasound stimulation group(8.53±3.43)%was significantly reduced on the third day after ultrasound stimulation compared with the MCAO group(23.45±1.99)%and the sham stimulation group(22.43±3.07)%(P<0.01).The mobility of the mice was improved on the fifth day after ultrasound stimulation,but there was no statistical difference among the three groups[MCAO group(1113.04±206.30)cm,sham stimulation group(944.08±206.60)cm,ultrasound stimulation group(1629.45±54.10)cm,P=0.068].Conclusions Low intensity focused ultrasound,as a neuromodulation technology,can reduce the brain damage of ischemic stroke and promote the recovery of motor function,and has great potential for the intervention of ischemic stroke.

关 键 词：低强度聚焦超声 神经调控技术 缺血性脑卒中 

分 类 号：R445.1[医药卫生—影像医学与核医学] R743.3[医药卫生—诊断学]