白细胞介素-33及其受体sST2在分泌性中耳炎中的表达及意义  

Expression of interleukin-33 and sST2 in secretory otitis media and its significance

作　　者：顾潇怡 马兆鑫[1] 王卫华[1] 郑炯[1] 崔佳文[1] 胡炯炯[1] 朱正华[1] 张茹 GU Xiaoyi;MA Zhaoxin;WANG Weihua;ZHENG Jiong;CUI Jiawen;HU Jiongjiong;ZHU Zhenghua;ZHANG Ru(Department of Otorhinolaryngology Head and Neck Surgery,Dongfang Hospital Affiliated to Tongji University,Shanghai 200120,China;Department of Otorhinolaryngology,Haining People’s Hospital in Zhejiang Province,Haining 314400,China)

机构地区：[1]同济大学附属东方医院耳鼻咽喉头颈外科,上海200120 [2]浙江省海宁市人民医院耳鼻咽喉头颈外科,海宁314400

出　　处：《中国眼耳鼻喉科杂志》2021年第2期114-116,共3页Chinese Journal of Ophthalmology and Otorhinolaryngology

基　　金：上海市浦东卫生和计划生育委员会重点专科疾病建设项目(PWZzb-2017-26)。

摘　　要：目的检测并分析分泌性中耳炎(SOM)患者中耳积液(MEE)中白细胞介素-33(IL-33)及其受体sST2的浓度。方法采用双抗夹心酶联免疫吸附法检测患者中耳积液中IL-33和sST2的浓度。结果慢性期组(病程≥3个月)MEE中IL-33含量明显高于急性期组(病程<3个月),差异具有统计学意义(P<0.05)。慢性期组MEE中sST2含量明显低于急性期组,差异具有统计学意义(P<0.05)。术后随访3个月,复发组IL-33、sST2含量与未复发组比较,差异无统计学意义。结论SOM慢性期IL-33呈现高表达,而sST2呈现低表达,提示IL-33/ST2信号通路激活及sST2表达下调在SOM慢性迁延过程中可能发挥重要作用。Objective To measure and analyze the level of IL-33 and its receptor sST2 in middle ear effusions(MEE)of patients with secretory otitis media(SOM).Methods The concentrations of IL-33 and sST2 in the middle ear effusions of patients were measured by enzyme-linked immunosorbent assay(ELISA).Results The levels of IL-33 in the middle ear effusion of chronic phase group(process≥3 months)were higher than those of the acute phase group(process<3 months),and the difference was statistically significant(P<0.05).The levels of sST2 in the middle ear effusion of chronic phase group were significantly lower than those of acute phase group,and the difference was statistically significant(P<0.05).After 3 months of post-operative followup,the levels of IL-33 and sST2 in the middle ear effusion had no significant differences between the recurrence group and the unrelapsed group.Conclusions IL-33 presents a high expression at chronic stage of secretory otitis media,while sST2 is low,suggesting that the activation of IL-33/ST2 signaling pathway and the down-regulation of sST2 may play an important role in the chronic delay of secretory otitis media.

关 键 词：分泌性中耳炎 白细胞介素-33 sST2 酶联免疫吸附试验 

分 类 号：R764.21[医药卫生—耳鼻咽喉科]