木薯C2H2型锌指蛋白转录因子家族全基因组鉴定及表达分析  

Genome-wide identification and expression analysis of C2H2-type zinc finger protein transcription factor family in cassava

作　　者：井建玲 张鹏 王振宇 马秋香 JING Jianling;ZHANG Peng;WANG Zhenyu;MA Qiuxiang(College of Tropical Crops,Hainan University,Haikou 570228,China;National Key Laboratory of Plant Molecular Genetics,Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences,Shanghai 200032,China;Institute of Bioengineering,Guangdong Academy of Sciences,Guangzhou 510316,China)

机构地区：[1]海南大学热带作物学院,海口570228 [2]中国科学院分子植物科学卓越创新中心,植物分子遗传国家重点实验室,上海200032 [3]广东省科学院生物工程研究所,广州510316

出　　处：《植物生理学报》2020年第12期2664-2676,共13页Plant Physiology Journal

基　　金：国家重点研发计划(2019YFD1001100);上海市自然科学基金(17ZR1435200);农业部国家现代农业产业技术体系(CARS-11-shzp)。

摘　　要：锌指蛋白转录因子(zinc-finger protein transcription factor, ZFP)是一种具有特定结构的蛋白,是真核生物重要转录因子之一,其通过与核内其他因子相互作用,调控各种生理反应,参与植物的生长发育和生物与非生物胁迫。目前对木薯锌指蛋白家族的研究依然较少。本研究利用木薯基因组数据库筛选鉴定了85个木薯C2H2型锌指蛋白转录因子家族成员,利用生物信息学手段分析该家族基因的结构特征和理化性质,结果表明同一C2H2型锌指蛋白家族的基因结构及氨基酸序列长度存在显著差异,大致可分为6个亚家族,各成员具有特征性的锌指结构域,但数量上存在差异;染色体定位显示该家族成员并非定位于木薯所有的18条染色体,在13号和16号染色体上没有C2H2型锌指蛋白家族成员定位。结合已有的转录组数据分析其在不同组织及非生物胁迫下的表达模式,发现各成员在不同组织中的表达模式不同,各组织均有高表达的基因,部分成员无组织表达差异,部分家族成员响应非生物胁迫。因此,木薯C2H2型锌指蛋白转录因子在植物生长发育和响应非生物胁迫功能方面具有重要的作用。Zinc finger protein transcription factors(ZFPs) are one of the important transcription factors in eukaryotes which contain a specific structure. ZFP usually interacts with other nuclear factors in order to regulate various physiological reactions, and participate in plant growth and development, and adaptation for biotic and abiotic stresses. C2 H2-type ZFPs not only bind to the DNA and RNA, but also interact with other proteins for their regulatory functions. However, there was less information on the C2 H2-type ZFPs in cassava. In this study, 85 members of C2 H2-type ZFPs were identified from the cassava genome database, and the structural characteristics and physicochemical properties of the family genes were analyzed by bioinformatics. Moreover, transcriptome profiles of C2 H2-type ZFPs were further investigated under different tissue and abiotic stresses from published transcriptome data. It showed that C2 H2-type ZFPs were divided into six subfamilies, and the gene structure and the length of amino acids were largely varied among the same C2 H2-type ZFPs. Each C2 H2-type ZFP subfamily contains a characteristic zinc finger domain, however, the amount of members were diverse for the six C2 H2-type ZFPs subfamilies. Furthermore, the C2 H2-type ZFP members was not even distributed on all 18 chromosomes of cassava while chromosome 13 and 16 did not have. Transcriptome analysis revealed that the transcript levels of each members were differentiated expressed in the tissues and under environmental stress conditions. Therefore, our study provides insight information on the functional characterization of C2 H2-type ZFPs in cassava.

关 键 词：木薯 C2H2型锌指蛋白转录因子 基因家族 生物信息学 

分 类 号：Q943.2[生物学—植物学] S533[农业科学—作物学]