Rice Protein Tagging Project:A Call for International Collaborations on Genome-wide In-Locus Tagging of Rice Proteins  

作　　者：Yuming Lu Pamela C.Ronald Bin Han Jiayang Li Jian-Kang Zhu 

机构地区：[1]Shanghai Center for Plant Stress Biology,CAS Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences,Shanghai,China [2]Department of Plant Pathology and the Genome Center,University of California,Davis,CA,USA [3]National Center for Gene Research,CAS Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences,Shanghai,China [4]State Key Laboratory of Plant Genomics,and National Center for Plant Gene Research(Beijing),Institute of Genetics and Developmental Biology,Innovation Academy for Seed Design,Chinese Academy of Sciences,Beijing,China [5]Department of Horticulture and Landscape Architecture,Purdue University,West Lafayette,IN,USA

出　　处：《Molecular Plant》2020年第12期1663-1665,共3页分子植物（英文版）

基　　金：the Chinese Academy of Sciences(to J.-K.Z.);the U.S.National Science Foundation Plant Genome Research Program,award no.2027795(to P.C.R.).

摘　　要：In September 1997,the International Rice Genome Sequencing Project was launched.This initiative pooled the resources of ten nations to obtain the first complete rice genome sequence,and promoted rice research and breeding into the post-genomics era(Li et al.,2018).In 2008,an internationally coordinated project named "RICE2020" was proposed to systematically and fully characterize all rice genes,transcripts,and proteins(Zhang et al.,2008).While genes and their transcripts can be readily characterized by sequencing-and PCR-based methods,the characterization of protein dynamics including protein levels,subcellular localizations,post-transla-tional modifications,and interactions with macromolecules(e.g.,proteins,DNA,RNA,carbohydrates,and lipids)and small molecules(e.g.,metabolites and ligands)is much more challenging and usually requires antibodies that specifically recognize the protein of interest.Because it is very difficult to systematically produce reliable antibodies for the specific recognition of individual plant proteins,a common practice is to transform a tag-fused open reading frame(ORF)of a gene to the corresponding loss-of-function mutant plants.However,such an ectopically expressed tagged protein may not fully reca-pitulate the properties of the endogenous protein due to the random insertion of the transgene,even when the transgene is expressed under the endogenous gene promoter.A preferred so-lution is to genetically label the coding sequence of the gene of interest,at its in vivo locus,with a sequence encoding a fluores-cent protein tag or an affinity tag such as FLAG or HA.Such "in-locus" protein tagging,as we are naming it here,has been carried out genome-wide in yeast,Caenorhabditis elegans,fly,and mammalian cultured cells,greatly facilitating the characterization of proteins in these organisms(Jiang et al.,2018).In 2017,a Genome Tagging Project was launched in mice,aiming to label every protein using a CRISPR/Cas9-based "artificial spermatids"method(Jiang et al.,2018).Significant funding and effor

关 键 词：BREEDING GENOME ENDOGENOUS 

分 类 号：S511[农业科学—作物学]