ζ-Carotene Isomerase Suppresses Tillering in Rice through the Coordinated Biosynthesis of Strigolactone and Abscisic Acid  

作　　者：Xue Liu Qingliang Hu Jijun Yan Kai Sun Yan Liang Meiru Jia Xiangbing Meng Shuang Fang Yiqin Wang Yanhui Jing Guifu Liu Dianxing Wu Chengcai Chu Steven M.Smith Jinfang Chu Yonghong Wang Jiayang Li Bing Wang 

机构地区：[1]State Key Laboratory of Plant Genomics,and National Center for Plant Gene Research(Beijing),Institute of Genetics and Developmental Biology,Innovation Academy for Seed Design,Chinese Academy of Sciences,Beijing 100101,China [2]University of Chinese Academy of Sciences,Beijing 100049,China [3]State Key Laboratory of Rice Biology,Institute of Nuclear Agriculture Sciences,Zhejiang University,Hangzhou 310029,China [4]School of Natural Sciences,University of Tasmania,Hobart 7001,Australia [5]State Key Laboratory of Plant Genomics,and National Center for Plant Gene Research(Beijing),Institute of Genetics and Developmental Biology,The CAS Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences,Beijing 100101,China [6]College of Life Sciences,Shandong Agricultural University,Taian 271018,China

出　　处：《Molecular Plant》2020年第12期1784-1801,共18页分子植物（英文版）

基　　金：grants from the Chinese Ministry of Science and Technology(2016YFD0100901);the Ministry of Agriculture of China(2016ZX08011001);the CAS President’s International Fellowship Initiative(2018VBA0025);the Youth Innovation Promotion Association CAS(2019099);the Strategic Priority Research Program of CAS(XDB27010100)(Beltran et al.,2015).

摘　　要：Rice tillering is an important agronomic trait affecting grain yield.Here,we identified a high-tillering mutant tillering20(t20),which could be restored to the wild type by treatment with the strigolactone(SL)analog rac-GR24.T20 encodes a chloroplast ζ-carotene isomerase(Z-ISO),which is involved in the biosynthesis of ca-rotenoids and their metabolites,SL and abscisic acid(ABA).The t20 mutant has reduced SL and ABA,raising the question of how SL and ABA biosynthesis is coordinated,and whether they have overlapping functions in tillering.We discovered that rac-GR24 stimulated T20 expression and enhanced all-trans-p-carotene biosynthesis.Importantly,rac-GR24 also stimulated expression of Oryza sativa 9-CIS-EPOXY-CAROTENOID DIOXYGENASE 1(OsNCED1)through induction of Oryza sativa HOMEOBOX12(0sHOX12),promoting ABA biosynthesis in shoot base.On the other hand,ABA treatment significantly repressed SL biosynthesis and the ABA biosynthetic mutants displayed elevated SL biosynthesis.ABA treatment reduced the number of basal tillers in both t20 and wild-type plants.Furthermore,while ABA-deficient mu-tants aba1 and aba2 had the same number of basal tillers as wild type,they had more unproductive upper tillers at maturity.This work demonstrates complex interactions in the biosynthesis of carotenoid,SLs and ABA,and reveals a role for ABA in the regulation of rice tillering.

关 键 词：rice(Oryza sativa L.) TILLERING ζ-carotene isomerase carotenoid STRIGOLACTONES abscisic acid 

分 类 号：S511[农业科学—作物学]